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Epigenetic Modification of Cloned Embryos Improves Nanog Reprogramming in Pigs

文献类型： 外文期刊

作者： Huan, Yanjun 1 ; Wang, Hongmei 1 ; Wu, Zhanfeng 3 ; Zhang, Jiguang 3 ; Zhu, Jiang 2 ; Liu, Zhonghua 2 ; He, Hongbin 1 ;

作者机构： 1.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan 250010, Shandong, Peoples R China

2.Northeast Agr Univ, Coll Life Sci, Haerbin 150030, Heilongjiang Pr, Peoples R China

3.Shouguang City Hosp Chinese Med, Weifang 262700, Shandong, Peoples R China

期刊名称：CELLULAR REPROGRAMMING （ 影响因子：1.987； 五年影响因子：1.887 ）

ISSN： 2152-4971

年卷期： 2015 年 17 卷 3 期

页码：

收录情况： SCI

摘要： Incomplete reprogramming of pluripotent genes in cloned embryos is associated with low cloning efficiency. Epigenetic modification agents have been shown to enhance the developmental competence of cloned embryos; however, the effect of the epigenetic modification agents on pluripotent gene reprogramming remains unclear. Here, we investigated Nanog reprogramming and the expression patterns of pluripotent transcription factors during early embryo development in pigs. We found that compared with fertilized embryos, cloned embryos displayed higher methylation in the promoter and 5'-untranslated region and lower methylation in the first exon of Nanog. When 5-aza-2'-deoxycytidine (5-aza-dC) or trichostatin A (TSA) enhanced the development of porcine cloned embryos, Nanog methylation reprogramming was also improved, similar to that detected in fertilized counterparts. Furthermore, our results showed that the epigenetic modification agents improved the expression levels of Oct4 and Sox2 and effectively promoted Nanog transcription in cloned embryos. In conclusion, our results demonstrated that the epigenetic modification agent 5-aza-dC or TSA improved Nanog methylation reprogramming and the expression patterns of pluripotent transcription factors, thereby resulting in the enhanced expression of Nanog and high development of porcine cloned embryos. This work has important implications in the improvement of cloning efficiency.

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