文献类型： 外文期刊
作者： Wang, Shuo 1 ; Li, Chen 1 ; Sun, Panpan 3 ; Shi, Jianli 1 ; Wu, Xiaoyan 1 ; Liu, Chang 1 ; Peng, Zhe 1 ; Han, Hong 1 ; Xu, S 1 ;
作者机构： 1.Inst Anim Sci & Vet Med Shandong Acad Agr Sci, Shandong Prov Key Lab Anim Dis Control & Breeding, Jinan, Peoples R China
2.Shandong Normal Univ, Coll Life Sci, Jinan, Peoples R China
3.Qingdao Agr Univ, Qingdao, Peoples R China
4.China Inst Vet Drug Control, Beijing, Peoples R China
关键词： apoptosis; porcine circovirus 2; PLC; IP3R; Cacpsdummy2+; endoplasmic reticulum stress
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： The endoplasmic reticulum (ER) plays an essential role in Ca2+ concentration balance and protein biosynthesis. During infection, the virus needs to complete its life process with the help of ER. At the same time, ER also produces ER stress (ERS), which induces apoptosis to resist virus infection. Our study explored the Ca2+ concentration, ERS, and the apoptosis mechanism after porcine circovirus 2 (PCV2) infection. We show here that PCV2 infection induces the increased cytoplasmic Ca2+ level and PK-15 cell ER swelling. The colocalization of phospholipase C (PLC) and inositol 1,4,5-trisphosphate receptor (IP3R) in the cytoplasm was observed by laser confocal microscopy. Western blot and quantitative polymerase chain reaction experiments confirmed that PLC and IP3R expression levels increased after PCV2 infection, and Ca2+ concentration in the cytoplasm increased after virus infection. These results suggest that PCV2 infection triggers ERS of PK-15 cells via the PLC-IP3R-Ca2+ signaling pathway to promote the release of intracellular Ca2+ and led to cell apoptosis.
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