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Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties

文献类型： 外文期刊

作者： Miao, Jun 1 ; Sun, Jinghan 1 ; Liu, Dongcheng 1 ; Li, Bin 1 ; Zhang, Aimin 1 ; Li, Zhensheng 1 ; Tong, Yiping 1 ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

2.Shandong Acad Agr Sci, Vegetable Res Inst, Jinan 250100, Peoples R China

关键词： high-affinity phosphate transporter;TaPHT1.2;promoter;differential expression;Triticum aestivum L.

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：4.275； 五年影响因子：5.223 ）

ISSN： 1673-8527

年卷期： 2009 年 36 卷 8 期

页码：

收录情况： SCI

摘要： TaPHT1.2 is a functional, root predominantly expressed and low phosphate (Pi) inducible high-affinity Pi transporter in wheat, which is more abundant in the roots of P-effieient wheat genotypes (e.g., Xiaoyan 54) than in P-inefficient genotypes (e.g., Jing 411) tinder both Pi-deficient and Pi-sufficient conditions. To characterize TaPHT1.2 further, we genetically mapped a TaPHT1.2 transporter, TaPHT1.2-D1, on the long arm of chromosome 4D using a recombinant inbred line Population derived from Xiaoyan 54 and Jing 411, and isolated a 1,302 bp fragment of the TaPHT1.2-D1 promoter (PrTaPHT1.2-D1) from Xiaoyan 54. TaPHT1.2-D1 shows collinearity with OsPHT1.2 that has previously been reported to mediate the translocation of Pi from roots to shoots. PrTaPHT1.2-D contains a number of Pi-starvation responsive elements, including P1BS, WRKY-binding W-box, and helix-loop-helix-binding elements. PrTaPHT1.2-D1 was then used to drive expression of beta-glucuronidase (GUS) reporter gene in Arabidopsis through Agrobacterium-mediated transformation. Histochemical analysis of transgenic Arabidopsis plants showed that the reporter gene was specifically induced by Pi-starvation and predominantly expressed in the roots. As there is only one SNP between the TaPHT1.2-D1 promoters of Xiaoyan 54 and Jing 411, and this SNP does not exist within the Pi-starvation responsive elements, the differential expression of TaPHT1.2 in Xiaoyan 54 and Jing 411 may not be caused by this SNP.

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