Latrophilin, an adhesion GPCR with galactose-binding lectin domain involved in the innate immune response of Tribolium castaneum
文献类型： 外文期刊
作者： Wang, Suisui 1 ; Bi, Jingxiu 1 ; Li, Chengjun 1 ; Li, Bin 1 ;
作者机构： 1.Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Biodivers & Biotechnol, Nanjing 210023, Peoples R China
2.Shandong Acad Agr Sci, Inst Qual Standard & Testing Technol Agroprod, Lab Qual & Safety Risk Assessment Agroprod, Minist Agr Jinan, Jinan, Shandong, Peoples R China
关键词： Latrophilin; Innate immunity; Pattern recognition receptor
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2023 年 253 卷
页码：
收录情况： SCI
摘要： Latrophilin is a member of adhesion GPCRs involved in various physiological pro1cesses. The extracellular fragment of Tribolium castaneum Latrophilin (TcLph) contains a galactose-binding lectin (GBL) domain. However, the biological function of GBL domain remains mysterious. Here, we initially studied the role of TcLph in recognizing pathogens through its GBL domain and then triggering immune defense in invertebrates. Results showed that GBL domain was highly conserved, and its predicted 3D structure was similar to rhamnose-binding lectin domain of mouse Latrophilin-1 with a unique & alpha;/& beta; fold and two long loops. Molecular docking and ELISA results revealed the GBL domain can bind to D-galactose, L-rhamnose, lipopolysaccharide and peptidoglycan. The recombinant extracellular segment of TcLph and the recombinant GBL exhibited strong agglutinating and binding activities to all tested bacteria in a Ca2+-dependent manner. Moreover, TcLph was markedly induced after infection by Escherichia coli or Staphylococcus aureus, while its silencing exacerbated bacterial loads and larvae mortality. TcLph-deficient larvae significantly decreased the transcription levels of antimicrobial peptides and prophenoloxidase activating system-related genes, leading to a significant reduction in phenoloxidase activity. It indicated that TcLph functioned as a pattern recognition receptor in pathogen recognition and activated immune responses to eliminate invasive microbes, suggesting a potential target for insecticides.
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