Partner of neuropeptide bursicon homodimer pburs mediates a novel antimicrobial peptide Ten3LP via Dif/Dorsal2 in Tribolium castaneum
文献类型： 外文期刊
作者： Li, Jingjing 1 ; Lyu, Bo 1 ; Bi, Jingxiu 1 ; Shan, Ruiqi 1 ; Stanley, David 1 ; Feng, Qili 4 ; Song, Qisheng 1 ;
作者机构： 1.Univ Missouri, Div Plant Sci & Technol, Columbia, MO 65211 USA
2.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Shandong, Peoples R China
3.US Dept Agr Agr Res Stn USDA ARS, Biol Control Insect Res Lab, Columbia, MO 65203 USA
4.South China Normal Univ, Inst Insect Sci & Technol, Sch Life Sci, Guangzhou Key Lab Insect Dev Regulat & Applicat Re, Guangzhou 510631, Peoples R China
关键词： Bursicon; Tribolium castaneum; Immune response; AMP genes; NF-KB; Tenecin 3 like peptide
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2023 年 247 卷
页码：
收录情况： SCI
摘要： Bursicon is a cystine knot family neuropeptide, composed of two subunits, bursicon (burs) and partner of burs (pburs). The subunits can form heterodimers to regulate cuticle tanning and wing maturation and homodimers to signal different biological functions in innate immunity, midgut stem cell proliferation and energy homeostasis, and reproductive physiology in the model insects Drosophila melanogaster or Tribolium castaneum. Here, we report on the role of the pburs homodimer in signaling innate immunity in T. castaneum larvae. Through transcriptome analysis we identified a set of immune-related genes that respond to pburs RNAi. Treating larvae with recombinant-pburs protein led to up-regulation of antimicrobial peptide (AMP) genes in vivo and in vitro. The upregulation of most AMP genes was dependent on the NF-KB transcription factor Relish. Most importantly, we identified a novel AMP, Tenecin 3-like peptide (Ten3LP), regulated by pburs via NF-KB transcription factor Dorsal-related immunity factor (Dif)/Dorsal2, but not Relish. We conducted Ten3LP RNAi, synthesized recombinant Ten3LP protein for microbial inhibition assays and functionally characterized Ten3LP as an AMP specific for fungi and Gram-positive bacteria. We demonstrate that expression of Ten3LP is activated by pburs via the Toll pathway. These findings identify new molecular targets for development of potential antibiotics for treating microbial infections and perhaps for RNAi based pest management technology.
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