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Development and validation of reverse transcription loop-mediated isothermal amplification for detection of PRRSV

文献类型： 外文期刊

作者： Chen, Changmu 1 ; Cui, Shangjin 1 ; Zhang, Chaofan 1 ; Li, Jun 2 ; Wang, Jinbao 2 ;

作者机构： 1.Harbin Vet Res Inst CAAS, Div Swine Infect Dis, State Key Lab Vet Biotechnol, Harbin 150001, Heilongjiang, Peoples R China

2.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China

关键词： Porcine reproductive and respiratory syndrome virus;Reverse-transcription loop-mediated isothermal amplification

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2010 年 40 卷 1 期

页码：

收录情况： SCI

摘要： To establish a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of porcine reproductive and respiratory syndrome virus (PRRSV), four primers specific to six regions of the N gene were designed. After amplification in an isothermal water bath for 1 h, samples containing PRRSV generated the expected ladder-like products while porcine parvovirus, porcine circovirus, classic swine fever virus, pseudorabies virus, and swine testis cells generated no product. The sensitivity and specificity of the RT-LAMP assay were evaluated by comparison with reverse-transcription polymerase chain reaction (RT-PCR) and real-time PCR. Because it is specific and simple, the RT-LAMP assay will be useful for the diagnosis of PRRSV infection.

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