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Construction and immunogenicity of DNA vaccines encoding fusion protein of murine complement C3d-p28 and GP5 gene of porcine reproductive and respiratory syndrome virus

文献类型： 外文期刊

作者： Zhang, Deqing 1 ; Xia, Qingxiang 1 ; Wu, Jiaqiang 3 ; Liu, Dong 4 ; Wang, Xiaolong 1 ; Niu, Zhongxiang 1 ;

作者机构： 1.Shandong Agr Univ, Coll Vet Med, Tai An 271018, Shandong, Peoples R China

2.Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China

3.Shandong Acad Agr Sci, Inst Vet Med, Jinan 250100, Shandong, Peoples R China

4.Jinan Anim Husb & Vet Bur, Jinan 250100, Shandong, Peoples R China

5.Taian Cent Hosp, Dept Lab, Tai An 271000, Shandong, Peoples R China

关键词： Complement C3d-p28;DNA vaccine;Molecular adjuvants;PRRSV GP5

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN： 0264-410X

年卷期： 2011 年 29 卷 4 期

页码：

收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) has recently caused catastrophic losses in swine industry worldwide. The commercial vaccines only provide a limited protection against PRRSV infection. At present, DNA vaccine is the focus on the new vaccines. The gene fragment (p28) coding for the molecular adjuvants complement protein C3d (mC3d) from BALB/c mouse was cloned and expressed as a fusion protein for its application in the vaccine study of mice. Three potential vaccines construct units were engineered to contain two, four and six copies of mC3d-p28 coding gene linked to the GP5 gene of PRRSV and one vaccine expressing GP5 alone (pcDNA3.1-GP5) was constructed. Subsequently, the vaccines' abilities to elicit the humoral and cellular immune responses were investigated in mice. These results showed that significantly enhanced GP5-specific ELISA antibody, GP5-specific neutralizing antibody, IFN-gamma level, and IL-4 level, could be induced in mice immunized with DNA construct units encoding the pcDNA3.1-C3d-p28.n-GP5 than those received DNA vaccine expressing GP5 alone (pcDNA3.1-GP5). Analysis of the immunogenicity of different repeats of mC3d-p28 revealed that mC3d-p28 had an enhancing effect on the immunogenicity of antigens, and that six or more repeats of mC3d-p28 may be necessary for efficient enhancement of antigen specific immune responses. This approach may provide a new strategy for the development of efficient vaccines against the PRRSV for pigs in the future. (C) 2010 Elsevier Ltd. All rights reserved.

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