您好，欢迎访问山东省农业科学院文献资源数据库平台

Rapid detection of newly isolated Tembusu-related Flavivirus by reverse-transcription loop-mediated isothermal amplification assay

文献类型： 外文期刊

作者： Wang, Youling 1 ; Yuan, Xiaoyuan 2 ; Li, Yufeng 2 ; Yu, Kexiang 2 ; Yang, Jinxing 2 ; Xu, Huaiying 2 ; Zhang, Yuxia 2 ; Y 1 ;

作者机构： 1.S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

2.Shandong Acad Agr Sci, Inst Poultry Sci, Jinan 250023, Peoples R China

3.Minist Agr Peoples Republ China, Beijing 100026, Peoples R China

关键词： Tembusu-related Flavivirus;newly-isolated virus;RT-PCR;RT-LAMP

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2011 年 8 卷

页码：

收录情况： SCI

摘要： Background: From April 2010 to January 2011, a severe new viral disease had devastated most duck-farming regions in China. This disease affected not only laying ducks but also meat ducks, causing huge economic losses for the poultry industry. The objective of this study is to develop a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of the new virus related to Tembusu-related Flavivirus. Results: The RT-LAMP assay is very simple and rapid, and the amplification can be completed within 50 min under isothermal conditions at 63 C by a set of 6 primers targeting the E gene based on the sequences analysis of the newly isolated viruses and other closely related Flavivirus. The monitoring of gene amplification can also be visualized by using SYBR green I fluorescent dye. In addition, the RT-LAMP assay for newly isolated Tembusu-related Flavivirus showed higher sensitivity with an RNA detection-limit of 2 copies/mu L compared with 190 copies/mu L of the conventional RT-PCR method. The specificity was identified without cross reaction to other common avian pathogens. By screening a panel of clinical samples this method was more feasible in clinical settings and there was higher positive coincidence rate than conventional RT-PCR and virus isolation. Conclusion: The RT-LAMP assay for newly isolated Tembusu-related Flavivirus is a valuable tool for the rapid and real-time detection not only in well-equipped laboratories but also in general conditions.

  • 相关文献

[1]Rapid detection of Prunus necrotic ringspot virus using magnetic nanoparticle-assisted reverse transcription loop-mediated isothermal amplification. Zong, Xiaojuan,Wang, Wenwen,Wei, Hairong,Wang, Jiawei,Chen, Xin,Xu, Li,Zhu, Dongzi,Tan, Yue,Liu, Qingzhong.

[2]INCIDENCE OF SWEET CHERRY VIRUSES IN SHANDONG PROVINCE, CHINA AND A CASE STUDY ON MULTIPLE INFECTION WITH FIVE VIRUSES. Zong, X.,Wang, W.,Wei, H.,Wang, J.,Liu, Q.,Hammond, R. W.,Yan, X..

[3]用复合RT-PCR方法诊断禽支原体与其他呼吸道病原的混合感染. 张秀美. 2003

[4]猪繁殖与呼吸综合征病毒山东株ORF7基因的克隆和鉴定. 吴家强,山东农业大学动科院,卢新存,山东农业大学动科院,李俊,王文志,黄艳艳,胡北侠,柳尧波,张秀美,王金宝. 2006

[5]应用RT-PCR和病毒学方法诊断山东省鸡新城疫感染的比较研究. 黄艳艳,胡北侠,张秀美,刘玉庆,卢新存,李俊. 2006

[6]马铃薯X病毒外壳蛋白基因的克隆及其原核表达载体的构建. 李广存,杨煜,王秀丽,高常勇,陈利容,毕玉平,王毅. 2002

[7]鸡传染性支气管炎山东A株病毒S_1高可变区基因的克隆. 孟兆宏,秦卓明,崔言顺,牛钟相,魏国红,杨萍萍. 2003

[8]一株韩国新型DHV的分离及RT-PCR鉴定. 马秀丽,赵立娜,夏雪梅,吴静,林树乾,姜亦飞. 2010

[9]融合蛋白C3d提高新城疫灭活苗的免疫原性. 李艳梅,褚新星,吴春涛,聂玉霞,肖连明. 2008

[10]槭树叶片类黄酮糖基转移酶基因RT-PCR扩增体系优化. 尹燕雷,苑兆和,招雪晴,冯立娟,杨尚尚,李云. 2013

[11]应用RT-PCR和病毒学方法诊断山东省鸡新城疫感染的比较研究. 黄艳艳,胡北侠,张秀美,刘玉庆,卢新存,李俊. 2007

[12]甜樱桃病毒病分子检测技术的研究. 宗晓娟,王文文,王甲威,魏海蓉,朱东姿,谭钺,刘庆忠. 2014

[13]1型鸭肝炎病毒基因组全长cDNA克隆的构建. 于可响,沙明利,林树乾,姜亦飞,黄兵. 2011

[14]花生子叶RNA提取方法比较与分析. 杨晨,徐凤花,单世华,刘宇,李春娟. 2011

[15]玉米ZmcpSRP54基因可变剪接分析. 关海英,何春梅,王娟,刘铁山,董瑞,刘春晓,刘强,汪黎明. 2019

[16]甘薯淀粉合成相关酶基因的克隆与表达分析. 侯夫云,李丰,秦桢,李爱贤,董顺旭,王庆美,张立明. 2019

[17]石榴花二氢黄酮醇还原酶(DFR)基因RT-PCR扩增体系的建立与优化. 招雪晴,苑兆和,陶吉寒,尹燕雷,冯立娟. 2011

[18]鹅星状病毒LAMP快速检测方法的建立与应用. 张玉霞,杨金兴,孟凯,蔡李萌,袁小远,王友令. 2019

[19]甜瓜果实酸性转化酶基因cDNA片段的克隆. 于喜艳,赵双宜,何启伟,孔庆国. 2003

[20]小麦TaLTR基因的克隆及初步表达分析. 徐平丽,彭振英,唐桂英,王燕龙,单雷. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台