您好，欢迎访问山东省农业科学院文献资源数据库平台

Cloning and Characterization of New Splice Variants of Insulin-Like Growth Factor-1 Gene in Songliao Black Pig

文献类型： 外文期刊

作者： Liu, Chang 1 ; Zhaoa, Zhihui 1 ; Bai, Wenlin 1 ; Sun, Boxing 1 ; Zhang, Yonghong 1 ; Yang, Runjun 1 ;

作者机构： 1.Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Jilin, Peoples R China

2.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Shandong, Peoples R China

3.Shenyang Agr Univ, Coll Anim Sci & Vet Med, Shenyang 110866, Liaoning, Peoples R China

关键词： Insulin-Like Growth Factor 1 (IGF-1);alternative splicing;Songliao Black pig;tissues;expression

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 17 期

页码：

收录情况： SCI

摘要： Insulin-Like Growth Factor 1 (IGF-1) is a single copy gene. It generates several IGF-1 mRNA variants due to alternative splicing. Spliced variants of IGF-1 being some small peptides derived from spliced variants of IGF-1 have critical roles in cell metabolism and growth of various vertebrate species. Porcine IGF-1 gene has two promoters and the existence of Exon 6 has been confirmed in Songliao Black pig in the laboratory. The aims of the present study were to identify new spliced variants of IGF-1 gene including Exon 5 and to investigate their expression profile in different tissues of Songliao Black pig. The cDNAs of two new spliced variants of IGF-1 gene were cloned by RACE. These two variants were named Class I IGF-1-Eb and Class II IGF-1-Eb, respectively. The Class I IGF-1-Eb contains an Open Reading Frame (ORE) of 564 bp encoding 187 amino acids (GenBank Accession No. FJ91 44971. The Class II IGF-1-Eb contains an ORE of 516-bp encodnig. 171 amino acids (GenBank Accession No. FJ91 4498). Tissue distributions of mRNAs of Class I IGF-1-Eb and Class II IGF-1-Eb were investigated in Songliao Black pig by semi-quantitative RT-PCR. Both of the new mRNA variants were expressed in a variety of tissues. Class I IGF-1-Eb mRNA was more abundant than Class II IGF-1-Eb mRNA in most tissues except for duodenum and longissimus muscle (p<0.05).

  • 相关文献

[1]A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression. Li, H.,Jiang, Q.,Ju, Z. H.,Huang, J. M.,Wang, X. G.,Yang, C. H.,Sun, Y.,Zhong, J. F.,Wang, C. F.,Li, H.,Yang, G. W.. 2016

[2]A SNP in intron 8 of CD46 causes a novel transcript associated with mastitis in Holsteins. Wang, Xiuge,Zhong, Jifeng,Gao, Yundong,Ju, Zhihua,Huang, Jinming,Huang, Jinming. 2014

[3]Increased expression of a novel splice variant of the complement component 4 (C4A) gene in mastitis-infected dairy cattle. Yang, Y.,Huang, J. M.,Ju, Z. H.,Li, Q. L.,Zhou, L.,Li, R. L.,Li, J. B.,Zhong, J. F.,Wang, C. F.,Yang, Y.,Shi, F. X.,Yang, Y.. 2012

[4]Two splice variants of the bovine lactoferrin gene identified in Staphylococcus aureus isolated from mastitis in dairy cattle. Huang, J. M.,Wang, Z. Y.,Ju, Z. H.,Wang, C. F.,Li, Q. L.,Sun, T.,Hou, Q. L.,Hang, S. Q.,Hou, M. H.,Zhong, J. F.. 2011

[5]Novel splice variants of the bovine PCK1 gene. Zhang, Z. B.,Zhao, Z. S.,Huang, J. M.,Zhang, Z. B.,Zhang, W.,Li, R. L.,Li, J. B.,Zhong, J. F.. 2013

[6]Alternative splicing and mRNA expression analysis of bovine SLAMF7 gene in healthy and mastitis mammary tissues. Ju, Zhihua,Wang, Changfa,Li, Qiuling,Hou, Minghai,Gao, Shuai,Hou, Qinlei,Li, Jianbin,Huang, Jinming,Zhong, Jifeng.

[7]Discovery of a new mechanism for regulation of plant triacylglycerol metabolism: The peanut diacylglycerol acyltransferase-1 gene family transcriptome is highly enriched in alternative splicing variants. Zheng, Ling,Guo, Feng,Shi, Lingmin,Li, Xinguo,Shan, Lei,Wan, Shubo,Peng, Zhenying,Shockey, Jay.

[8]Differential expression of two novel odorant receptors in the locust (Locusta migratoria). Xu, Haozhi,Guo, Mei,Yang, Ying,You, Yinwei,Zhang, Long,You, Yinwei. 2013

[9]Genome-wide identification and analysis of the growth-regulating factor family in Chinese cabbage (Brassica rapa L. ssp pekinensis). Wang, Fengde,Ding, Qian,Li, Jingjuan,Zhang, Yihui,Li, Huayin,Gao, Jianwei,Qiu, Nianwei. 2014

[10]Transgenic Expression and Identification of Recombinant Human Proinsulin in Peanut. Zheng Ling,Wang Yu,Wan Shu-Bo,Peng Zhen-Ying,Bi Yu-Ping,Zheng Ling,Wang Yu,Bian Fei,Wan Shu-Bo,Peng Zhen-Ying,Zheng Ling,Wang Yu,Bian Fei,Wan Shu-Bo,Peng Zhen-Ying,Bi Yu-Ping,Jiao Qi-Qing,Qu Shu-Jie,Wan Shu-Bo,Bi Yu-Ping. 2016

[11]Molecular analysis and recombinant expression of bovine neutrophil beta-defensin 12 and its antimicrobial activity. Wu, Jianming,Wang, Changfa,He, Hongbin,Yang, Hongjun,Gao, Yundong,Zhong, Jifeng,Hu, Guixue. 2011

[12]Cloning and Expression Analysis of Eight Upland Cotton Pentatricopeptide Repeat Family Genes. Han, Zongfu,Kong, Fanjin,Deng, Yongsheng,Wang, Zongwen,Shen, Guifang,Wang, Jinghui,Duan, Bing,Li, Ruzhong,Qin, Yuxiang.

[13]Characterization and biocontrol ability of fusion chitinase in Escherichia coli carrying chitinase cDNA from Trichothecium roseum. Pan, Hongyu,Wei, Yi,Xin, Furong,Zhou, Mingguo,Zhang, Shihong.

[14]Characterization and expression analysis of a novel RING-HC gene, ZmRHCP1, involved in brace root development and abiotic stress responses in maize. Li Wen-lan,Sun Qi,Li Wen-cai,Yu Yan-li,Zhao Meng,Meng Zhao-dong. 2017

[15]Molecular characterization and chromosomal mapping of porcine brain and muscle Arnt-like protein-1 gene. Xing, Jinyi,Xu, Qinying,Jiang, Yunliang,Li, Kui,Wang, Jiying,Wu, Ying,Xing, Jinyi.

[16]Genomic organization and expression analysis of a farnesyl diphosphate synthase gene (FPPS2) in apples (Malus domestica Borkh.). Yuan, Kejun,Wang, Changjun,Xin, Li,Zhang, Anning,Ai, Chengxiang.

[17]Molecular cloning, expression, and evolution analysis of type II CHI gene from peanut (Arachis hypogaea L.). Liu, Yu,Zhao, Shuzhen,Wang, Jiangshan,Zhao, Chuanzhi,Hou, Lei,Li, Changsheng,Xia, Han,Guan, Hongshan,Wang, Xingjun,Liu, Yu,Wang, Xingjun.

[18]Novel SNPs in HSP70A1A gene and the association of polymorphisms with thermo tolerance traits and tissue specific expression in Chinese Holstein cattle. Li, Qiuling,Han, Jianbo,Du, Fanglei,Ju, Zhihua,Huang, Jinming,Wang, Ji,Li, Rongling,Wang, Changfa,Zhong, Jifeng.

[19]High level resistance to Turnip mosaic virus in Chinese cabbage (Brassica campestris ssp pekinensis (Lour) Olsson) transformed with the antisense NIb gene using marker-free Agrobacterium tumefaciens infiltration. Yu Zhandong,Zhao Shuangyi,He Qiwei.

[20]Molecular cloning and expression analysis of CmMlo1 in melon. Cheng, Hong,Kun, Weiping,Liu, Dongshun,Su, Yongquan,He, Qiwei.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台