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Concurrent highly pathogenic porcine reproductive and respiratory syndrome virus infection accelerates Haemophilus parasuis infection in conventional pigs

文献类型： 外文期刊

作者： Yu, Jiang 2 ; Wu, Jiaqiang 1 ; Zhang, Yuyu 1 ; Guo, Lihui 1 ; Cong, Xiaoyan 1 ; Du, Yijun 1 ; Li, Jun 1 ; Sun, Wenbo 1 ; Shi 1 ;

作者机构： 1.Shandong Acad Agr Sci, Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China

2.Shandong Normal Univ, Jinan, Peoples R China

关键词： Effect;Co-infection;HP-PRRSV;Haemophilus parasuis;Quantitative real-time PCR

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2012 年 158 卷 3-4 期

页码：

收录情况： SCI

摘要： This study was aimed at determining the effect of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) on Haemophilus parasuis (HPS) in co-infection. A quantitative real-time PCR targeting infB gene, which is conserved among different HPS serotypes, was developed to improve the accuracy and speed of the detection of HPS. A total of 32 four-week-old conventional pigs were distributed randomly into four groups: pigs in group I were intranasally infected with HP-PRRSV first, and were then intraperitoneally inoculated with HPS on 5 days after HP-PRRSV infection; pigs in group II were intranasally inoculated with HP-PRRSV alone; pigs in group III were intraperitoneally inoculated with HPS alone; pigs in group IV were intraperitoneally inoculated with physiological saline. The amount of HPS in serum on 0, 3, 6, 9 and 12 days post-inoculation (dpi) with HPS were detected using the established quantitative real-time PCR. Clinical signs, pathological changes and histopathological lesions were observed. The amount of HPS in serum reached 10(6) copies/mu l at 3 dpi with HPS in pigs of group I, while it arrived 10(5.7) copies/mu l at 9 dpi with HPS in pigs of group III. The HPS loads in hearts and lungs were much higher than in other tissues. The study showed that HP-PRRSV was able to accelerate BPS infection and loads. (C) 2012 Elsevier B.V. All rights reserved.

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