您好，欢迎访问山东省农业科学院文献资源数据库平台

A comprehensive microRNA expression profile of the backfat tissue from castrated and intact full-sib pair male pigs

文献类型： 外文期刊

作者： Bai, Ying 1 ; Huang, Jin-Ming 1 ; Liu, Gang 1 ; Zhang, Ji-Bin 1 ; Wang, Jian-Ying 3 ; Liu, Cheng-Kun 1 ; Fang, Mei-Yin 1 ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, MOA Lab Anim Genet & Breeding, Dept Anim Genet & Breeding,Natl Engn Lab Anim Bre, Beijing 100193, Peoples R China

2.Shandong Acad Agr Sci, Jinan 250131, Peoples R China

3.Shandong Acad Agr Sci, Jinan 25013

关键词： Male pig;MicroRNA;Fat deposition;Castration

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2014 年 15 卷

页码：

收录情况： SCI

摘要： Background: It is widely known that castration has a significant effect on the accumulation of adipose tissue. microRNAs (miRNAs) are known to be involved in fat deposition and to be regulated by the androgen-induced androgen receptor (AR). However, there is little understanding of the relationship between miRNAs and fat deposition after castration. In this study, the high-throughput SOLiD sequencing approach was used to identify and characterize miRNA expression in backfat from intact and castrated full-sib male 23-week-old pigs. The patterns of adipogenesis and fat deposition were compared between castrated and intact male pigs. Results: A total of 366 unique miRNA genes were identified, comprising 174 known pre-miRNAs and 192 novel pre-miRNAs. One hundred and sixty-seven pre-miRNAs were common to both castrated (F3) and intact (F4) male pig small RNA libraries. The novel pre-miRNAs encoded 153 miRNAs/miRNA* s and 141 miRNAs/miRNA* s in the F3 and F4 libraries, respectively. One hundred and seventy-seven miRNAs, including 45 up-and 132 down-regulated, had more than 2-fold differential expression between the castrated and intact male pigs (p-value < 0.001). Thirty-five miRNAs were further selected, based on the expression abundance and differentiation between the two libraries, to predict their targets in KEGG pathways. KEGG pathway analyses suggested that miRNAs differentially expressed between the castrated and intact male pigs are involved in proliferation, apoptosis, differentiation, migration, adipose tissue development and other important biological processes. The expression patterns of eight arbitrarily selected miRNAs were validated by stem-loop reverse-transcription quantitative polymerase chain reaction. These data confirmed the expression tendency observed with SOLiD sequencing. miRNA isomiRs and mirtrons were also investigated in this study. Mirtrons are a recently described category of miRNA relying on splicing rather than processing by the microprocessor complex to generate the RNAi pathway. The functions of miRNAs important for regulating fat deposition were also investigated in this study. Conclusions: This study expands the number of fat-deposition-related miRNAs in pig. The results also indicate that castration can significantly affect the expression patterns of fat-related miRNAs. The differentially expressed miRNAs may play important roles in fat deposition after castration.

  • 相关文献

[1]中国荷斯坦牛HSF1基因microRNA SNPs与耐热性能的相关性研究. 李秋玲,鞠志花,贾祥捷,黄金明,李建斌,李荣岭,李芳,王长法,仲跻峰. 2011

[2]奶牛OLR1 3'UTR A223C多态的生物学信息分析. 张传生,耿立英,尹春光,曹顶国,刘铮铸,付志新. 2011

[3]4条小麦保守MicroRNAs的表达谱分析及其靶基因预测. 闫妍,韩冉,赵惠贤. 2012

[4]miRBase-microRNA序列数据库. 高青,鞠志花,王长法,李国荣. 2011

[5]鸡microRNA基因“seed”区4个SNPs的群体遗传分布. 耿立英,张传生,尹春光,曹顶国,付志新. 2011

[6]奶牛OLR1 3′UTR A223C多态的生物学信息分析(英文). 张传生,耿立英,尹春光,曹顶国,刘铮铸,付志新. 2010

[7]High-throughput sequencing discovery of conserved and novel microRNAs in Chinese cabbage (Brassica rapa L. ssp pekinensis). Wang, Fengde,Li, Libin,Liu, Lifeng,Li, Huayin,Zhang, Yihui,Gao, Jianwei,Yao, Yingyin,Ni, Zhongfu. 2012

[8]Genome-wide identification of microRNAs and their targets in wild type and phyB mutant provides a key link between microRNAs and the phyB-mediated light signaling pathway in rice. Sun, Wei,wu, Xiu,Xie, Xianzhi,Xu, Xiao Hui,Lu, Xingbo,Sun, Hongwei,Wang, Yong. 2015

[9]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

[10]Solexa sequencing of novel and differentially expressed microRNAs in testicular and ovarian tissues in Holstein Cattle. Huang, Jinming,Ju, Zhihua,Li, Qiuling,Hou, Qinlei,Wang, Changfa,Li, Jianbin,Li, Rongling,Wang, Lingling,Sun, Tao,Hang, Suqin,Gao, Yundong,Hou, Minghai,Zhong, Jifeng.

[11]Virus-Based MicroRNA Silencing and Overexpressing in Common Wheat (Triticum aestivum L.). Jian, Chao,Chi, Qing,Wang, Shijuan,Ma, Meng,Liu, Xiangli,Zhao, Huixian,Han, Ran,Zhao, Huixian. 2017

[12]microRNA/microRNA* complementarity is important for the regulation pattern of NFYA5 by miR169 under dehydration shock in Arabidopsis. Du, Qingguo,Gao, Wei,Sun, Suzhen,Li, Wen-Xue,Zhao, Meng. 2017

[13]MicroRNA expression analysis of rosette and folding leaves in Chinese cabbage using high-throughput Solexa sequencing. Wang, Fengde,Li, Huayin,Zhang, Yihui,Li, Jingjuan,Li, Libin,Liu, Lifeng,Wang, Lihua,Wang, Cuihua,Gao, Jianwei.

[14]Response of microRNAs to cold treatment in the young spikes of common wheat. Song, Guoqi,Zhang, Rongzhi,Zhang, Shujuan,Li, Yulian,Gao, Jie,Han, Xiaodong,Chen, Mingli,Wang, Jiao,Li, Wei,Li, Genying,Song, Guoqi,Zhang, Rongzhi,Zhang, Shujuan,Li, Yulian,Gao, Jie,Han, Xiaodong,Chen, Mingli,Wang, Jiao,Li, Wei,Li, Genying,Song, Guoqi,Zhang, Rongzhi,Zhang, Shujuan,Li, Yulian,Gao, Jie,Han, Xiaodong,Chen, Mingli,Wang, Jiao,Li, Wei,Li, Genying. 2017

[15]Mining, identification and function analysis of microRNAs and target genes in peanut (Arachis hypogaea L.). Zhang, Tingting,Hu, Shuhao,Yan, Caixia,Li, Chunjuan,Zhao, Xiaobo,Shan, Shihua,Hu, Shuhao,Wan, Shubo.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台