Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex-sorted bull sperm by inhibiting the oxidative stress
文献类型： 外文期刊
作者： Zhang, Pei-Pei 1 ; Wang, Jing-Jing 1 ; Li, Chong-Yang 1 ; Hao, Hai-sheng 1 ; Wang, Hao-Yu 1 ; Du, Wei-Hua 1 ; Pang, Yun-Wei 1 ; Zhao, Shan-Jiang 1 ; Zou, Hui-Ying 1 ; Hao, Tong 1 ; Yang, Sha 1 ; Ruan, Wei-Min 3 ; Huang, Jin-Ming 4 ; Jiao, Peng-cheng 5 ; Zhu, Hua-Bin 1 ; Zhao, Xue-Ming 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Embryo Biotechnol & Reprod Lab, 2 Yuanmingyuan Western Rd, Beijing 100193, Peoples R China
2.Chinese Acad Agr Sci, Inst Anim Sci, Ctr Domest Anim Reprod & Breeding, 2 Yuanmingyuan Western Rd, Beijing 100193, Peoples R China
3.Henan Univ, Int Joint Ctr Biomed Innovat, Sch Life Sci, Kaifeng, Peoples R China
4.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan, Peoples R China
5.Tsinghua Univ, Ctr Biomed Anal, Beijing, Peoples R China
关键词： in vitro fertilization; oxidative stress; sexual selection; sperm
期刊名称：REPRODUCTION IN DOMESTIC ANIMALS （ 影响因子：2.005； 五年影响因子：2.154 ）
ISSN： 0936-6768
年卷期： 2020 年 55 卷 9 期
页码：
收录情况： SCI
摘要： The fertilization capacity of sex-sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex-sorted bull sperm. In this study, the washing medium and fertilization medium of sex-sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1 x 10(-3), 1 x 10(-4), 1 x 10(-5), 1 x 10(-6) M) or Lyc (0, 1 x 10(-4), 1 x 10(-5), 1 x 10(-6), 1 x 10(-7)). After washing twice and incubation for 1.5 hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (Delta psi m) and IVF (in vitro fertilization) ability of sex-sorted sperm were investigated. For the sex-sorted sperm of bulls A, B and C, 1 x 10(-3) M VC or 1 x 10(-4) M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their Delta psi m levels and cleavage rates after IVF. When blastocysts were concerned, 1 x 10(-4) M Lyc significantly improved the blastocyst rates and their IFN-tau expression of bulls A and C. In conclusion, supplementation of 1 x 10(-3) M VC or 1 x 10(-4) M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex-sorted bull sperm during IVF procedure.
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