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Identification and Functional Analysis of Chitin Synthase A in Oriental Armyworm, Mythimna separata

文献类型： 外文期刊

作者： Zhai, Yifan 1 ; Fan, Xiaobin 2 ; Yin, Zhenjuan 1 ; Yue, Xiangzhao 2 ; Men, Xingyuan 1 ; Zheng, Li 1 ; Zhang, Wenqing 2 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Plant Protect, Jinan, Shandong, Peoples R China

2.Sun Yat Sen Univ, State Key Lab Biocontrol, Guangzhou, Guangdong, Peoples R China

3.Sun Yat Sen Univ, Sch Life Sci, Guangzhou, Guangdong, Peoples R China

关键词： chitin synthases;ecdysis;Mythimna separata;oogenesis;RNAi

期刊名称：PROTEOMICS （ 影响因子：3.984； 五年影响因子：3.831 ）

ISSN： 1615-9853

年卷期： 2017 年 17 卷 21 期

页码：

收录情况： SCI

摘要： Chitin synthases are very important enzymes for chitin synthesis in various species, which makes them a specific target of insecticides. In the present study, the function of the chitin synthase A (CHSA) gene isolated from Mythimna separate is investigated. The majority of dsMysCHSA treated larvae (89.50%) exhibit lethal phenotypes, including three phenotypes with severe cuticle deformations. The dsMysCHSA treatment in adult females affects oogenesis, and significantly reduce the ovary size and the oviposition number compared with controls. To determine how MysCHSA affects female fecundity, combined analyses of RNA-sequencing (RNA-Seq) transcriptome and TMT proteome (tandem mass tags) data in M. separata after treatment with MysCHSA-RNAi is performed. The differentially expressed proteins and genes affect fecundity-related proteins, energy metabolism, fatty acid metabolism, amino sugars, and nucleotide sugar metabolism pathways. Taken together, these results suggest that MysCHSA acts on M. separata ecdysis and fecundity, and has the potential as a target gene for pest control.

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