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Production of an aberrant splice variant of CCL5 is not caused by genetic mutation in the mammary glands of mastitis-infected Holstein cows

文献类型： 外文期刊

作者： Yang, Ling 1 ; Guo, Ruiqing 1 ; Ju, Zhihua 2 ; Wang, Xiuge 2 ; Jiang, Qiang 2 ; Liu, Yong 1 ; Zhao, Han 2 ; He, Kaili 1 ; Li, Jianbin 2 ; Huang, Jinming 2 ;

作者机构： 1.Hebei Univ Engn, Coll Life Sci & Food Engn, Handan 056021, Hebei, Peoples R China

2.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, 159 Ind North Rd, Jinan 250100, Shandong, Peoples R China

3.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Shandong, Peoples R China

关键词： alternative splicing; bovine mastitis; C-C motif chemokine ligand 5; single nucleotide polymorphisms

期刊名称：MOLECULAR MEDICINE REPORTS （ 影响因子：2.952； 五年影响因子：2.754 ）

ISSN： 1791-2997

年卷期： 2019 年 19 卷 5 期

页码：

收录情况： SCI

摘要： Genetic mutations, including single nucleotide polymorphisms (SNPs), result in aberrant alternatively splicing of gene and involves in susceptibility of inflammatory diseases, including bovine mastitis. C-C motif chemokine ligand 5 (CCL5) is an immune-associated gene, but its alternative splicing (AS) mechanism of gene expression has not yet been understood. The present study identified the splice variant of CCL5 and the compared differential expression of various transcripts between healthy and mastitic mammary gland tissue from cows. A novel transcript lacking exon 2 with a deletion of 112 bp (referred to as CCL5-AS) was identified in the mammary gland. The expression of CCL5-AS was lower compared with CCL5-reference in the healthy and mastitic mammary tissues. A total of two novel SNPs (g.1647 C>T and g.1804 G>A) were identified in exon 2 of CCL5. Using the splicing mini-gene reporter assay in bovine mammary epithelial cells (MAC-T) and 293T cells, it was confirmed that the production of CCL5-AS was not caused by the two SNPs. The present findings suggested that alternative splicing is one of the mechanisms of CCL5 expression regulation and is involved in mastitis infection, but that genetic mutation was not responsible for the generation of the abnormal transcript of CCL5 in cows.

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[1]Production of an aberrant splice variant of CCL5 is not caused by genetic mutation in the mammary glands of mastitis-infected Holstein cows. Yang, Ling,Guo, Ruiqing,Liu, Yong,He, Kaili,Guo, Ruiqing,Ju, Zhihua,Wang, Xiuge,Jiang, Qiang,Liu, Yong,Zhao, Han,Li, Jianbin,Huang, Jinming,Zhao, Han,Huang, Jinming. 2019

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