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Comparative Transcriptome and Co-Expression Network Analyses Reveal the Molecular Mechanism of Calcium-Deficiency-Triggered Tipburn in Chinese Cabbage (Brassica rapa L. ssp. Pekinensis)

文献类型： 外文期刊

作者： Zhang, Shu 1 ; Gao, Hanzhong 2 ; Wang, Lixia 1 ; Zhang, Yihui 1 ; Zhou, Dandan 3 ; Anwar, Ali 1 ; Li, Jingjuan 1 ; Wang, Fengde 1 ; Li, Cheng 1 ; Zhang, Ye 4 ; Gao, Jianwei 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Vegetables, Shandong Branch Natl Vegetable Improvement Ctr, Shandong Key Lab Greenhouse Vegetable Biol,Huanghu, Jinan 250100, Peoples R China

2.George Washington Univ, Columbian Coll Arts & Sci, Phillips Hall, 801 22nd St NW, Washington, DC 20052 USA

3.Shandong Normal Univ, Coll Life Sci, Jinan 250061, Peoples R China

4.Huangshan Univ, Coll Life Sci, Huangshan 245061, Peoples R China

关键词： Chinese cabbage; calcium deficiency; tipburn; RNA-seq; co-expression analysis

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

ISSN：

年卷期： 2022 年 11 卷 24 期

页码：

收录情况： SCI

摘要： Chinese cabbage tipburn is characterized by the formation of necrotic lesions on the margin of leaves, including on the insides of the leafy head. This physiological disorder is associated with a localized calcium deficiency during leaf development. However, little information is available regarding the molecular mechanisms governing Ca-deficiency-triggered tipburn. This study comprehensively analysed the transcriptomic comparison between control and calcium treatments (CK and 0 mM Ca) in Chinese cabbage to determine its molecular mechanism in tipburn. Our analysis identified that the most enriched gene ontology (GO) categories are photosynthesis, thylakoid and cofactor binding. Moreover, the KEGG pathway was most enriched in photosynthesis, carbon metabolism and carbon fixation. We also analyzed the co-expression network by functional categories and identified ten critical hub differentially expressed genes (DEGs) in each gene regulatory network (GRN). These DEGs might involve abiotic stresses, developmental processes, cell wall metabolism, calcium distribution, transcription factors, plant hormone biosynthesis and signal transduction pathways. Under calcium deficiency, CNX1, calmodulin-binding proteins and CMLs family proteins were downregulated compared to CK. In addition, plant hormones such as GA, JA, BR, Auxin and ABA biosynthesis pathways genes were downregulated under calcium treatment. Likewise, HATs, ARLs and TCP transcription factors were reported as inactive under calcium deficiency, and potentially involved in the developmental process. This work explores the specific DEGs' significantly different expression levels in 0 mM Ca and the control involved in plant hormones, cell wall developments, a light response such as chlorophylls and photosynthesis, transport metabolism and defence mechanism and redox. Our results provide critical evidence of the potential roles of the calcium signal transduction pathway and candidate genes governing Ca-deficiency-triggered tipburn in Chinese cabbage.

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[1]Comparative Transcriptome and Co-Expression Network Analyses Reveal the Molecular Mechanism of Calcium-Deficiency-Triggered Tipburn in Chinese Cabbage (Brassica rapa L. ssp. Pekinensis). Zhang, Shu,Wang, Lixia,Zhang, Yihui,Anwar, Ali,Li, Jingjuan,Wang, Fengde,Li, Cheng,Gao, Jianwei,Gao, Hanzhong,Zhang, Yihui,Zhou, Dandan,Zhang, Ye. 2022

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