PHB3 interacts with BRI1 and BAK1 to mediate brassinosteroid signal transduction in Arabidopsis and tomato
文献类型： 外文期刊
作者： Li, Cheng 1 ; Zhang, Shan 1 ; Li, Jingjuan 1 ; Huang, Shuhua 2 ; Zhao, Tong 2 ; Lv, Siqi 2 ; Liu, Jianwei 2 ; Wang, Shufen 2 ; Liu, Xiaohui 5 ; He, Shen 5 ; Zhang, Yanfeng 4 ; Xiao, Fangming 6 ; Wang, Fengde 1 ; Gao, Jianwei 1 ; Wang, Xiaofeng 2 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Vegetables, Jinan 250100, Peoples R China
2.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China
3.Shandong Inst Innovat & Dev, Jinan 250101, Peoples R China
4.Hybrid Rapeseed Res Ctr Shaanxi Prov, Yangling 712100, Shaanxi, Peoples R China
5.Xian Highness Agr Sci & Technol Co Ltd, Xian 710086, Shaanxi, Peoples R China
6.Univ Idaho, Dept Plant Sci, Moscow, ID 83844 USA
关键词： Arabidopsis; brassinosteroid; BRASSINOSTEROID-INSENSITIVE 1; BRI1 ASSOCIATED RECEPTOR KINASE 1; phosphorylation; PROHIBITIN 3; tomato
期刊名称：NEW PHYTOLOGIST （ 影响因子：9.4； 五年影响因子：10.5 ）
ISSN： 0028-646X
年卷期： 2024 年 241 卷 4 期
页码：
收录情况： SCI
摘要：
Brassinosteroids (BRs) are plant hormones that are essential in plant growth and development. BRASSINOSTEROID-INSENSITIVE 1 (BRI1) and BRI1 ASSOCIATED RECEPTOR KINASE 1 (BAK1), which are located on the plasma membrane, function as co-receptors that accept and transmit BR signals.PROHIBITIN 3 (PHB3) was identified in both BRI1 and BAK1 complexes by affinity purification and LC-MS/MS analysis. Biochemical data showed that BRI1/BAK1 interacted with PHB3 in vitro and in vivo.
BRI1/BAK1 phosphorylated PHB3 in vitro. When the Thr-80 amino acid in PHB3 was mutated to Ala, the mutant protein was not phosphorylated by BRI1 and the mutant protein interaction with BRI1 was abolished in the yeast two-hybrid assay. BAK1 did not phosphorylate the mutant protein PHB3(T54A). The loss-of-function phb3 mutant showed a weaker BR signal than the wild-type. Genetic analyses revealed that PHB3 is a BRI1/BAK1 downstream substrate that participates in BR signalling. PHB3 has five homozygous in tomato, and we named the closest to AtPHB3 as SlPHB3.1. Biochemical data showed that SlBRI1/SlSERK3A/SlSERK3B interacted with SlPHB3.1 and SlPHB3.3. The CRISPR-Cas9 method generated slphb3.1 mutant led to a BR signal stunted relatively in tomatoes.
PHB3 is a new component of the BR signal pathway in both Arabidopsis and tomato.PHB3 is a new component of the BR signal pathway in both Arabidopsis and tomato.
- 相关文献
作者其他论文 更多>>
-
Development of an analytic method for organosulfur compounds in Welsh onion and its use for nutritional quality analysis of five typical varieties in China
作者：Liu, Pingxiang;Bi, Jingxiu;Jiang, Yuying;Gao, Rui;Gao, Lei;Zhao, Tong;Zhang, Xiao;Zhang, Chao;Wang, Yutao;Wu, Peng;Li, Yonghua
关键词：Enzymatic inhibition; UHPLC-MS/MS; Organosulfur compound; Amino acid; Nutritional quality
-
BrMYB116 transcription factor enhances Cd stress tolerance by activating FIT3 in yeast and Chinese cabbage
作者：Anwar, Ali;Yuan, Chao;Cui, Bing;Wang, Lixia;He, Lilong;Gao, Jianwei;Anwar, Ali;Yuan, Chao;Yuan, Chao
关键词：Chinese cabbage; abiotic stress; BrMYB116; FIT3; Cd stress; RNA-seq
-
Silencing gram-negative bacteria binding protein 1 decreases the immunity of Tribolium castaneum against bacteria
作者：Bi, Jingxiu;Liu, Pingxiang;Gao, Rui;Jiang, Yuying;Zhang, Chao;Zhao, Tong;Gao, Lei;Wang, Yutao;Wang, Yutao
关键词：Tribolium castaneum; Gram-negative bacteria binding protein; Innate immunity; Molecular docking; Pest management
-
Genome-Wide Identification, Expression Analysis and Functional Study of DELLA Genes in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)
作者：Yuan, Yongxue;Wang, Lixia;Liu, Chenwen;Fu, Xin;Li, Xiaolong;Qiu, Minghui;Li, Jingjuan;Zhang, Yihui;Li, Cheng;Wang, Fengde;Gao, Jianwei;Yuan, Yongxue;Qiu, Nianwei;Zhao, Qianyu;Liu, Chenwen;Qiu, Minghui
关键词：Brassica rapa; DELLA protein; evolution; stress tolerance; growth and development
-
The Roles of Gibberellins in Regulating Leaf Development
作者：Ritonga, Faujiah Nurhasanah;Zhou, Dandan;Zhang, Yihui;Li, Cheng;Li, Jingjuan;Gao, Jianwei;Ritonga, Faujiah Nurhasanah;Zhou, Dandan;Song, Runxian
关键词：bioactive; biosynthesis; crosstalk; metabolism; phytohormones
-
Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
作者：Wang, Lixia;Zhang, Shu;Li, Jingjuan;Zhang, Yihui;Zhou, Dandan;Li, Cheng;He, Lilong;Li, Huayin;Wang, Fengde;Gao, Jianwei;Zhang, Ye;Zhou, Dandan
关键词：leaf size; cell cycle; cyclins; MYB transcription factor; Chinese cabbage
-
BrCYP71A15 Negatively Regulates Hg Stress Tolerance by Modulating Cell Wall Biosynthesis in Yeast
作者：Anwar, Ali;Zhang, Shu;Wang, Lixia;He, Lilong;Gao, Jianwei
关键词：Chinese cabbage; Hg stress; GFP; yeast; cell wall; gene expression
