Rapid quantitative detection of capsaicinoids in serum based on an electrochemical immunosensor with a dual-signal amplification strategy
文献类型： 外文期刊
作者： Zhao, Qingxue 1 ; Wu, Yuxiang 4 ; Shi, Xiaojie 1 ; Dong, Haowei 1 ; Liu, Huimin 1 ; Zheng, Yan 5 ; Yang, Qingqing 1 ; Sun 1 ;
作者机构： 1.Shandong Univ Technol, Sch Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.Shandong Prov Engn Res Ctr Vegetable Safety & Qua, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
3.Zibo City Key Lab Agr Prod Safety Traceabil, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
4.Shandong Lvdu Biosci & Technol Co Ltd, Binzhou 256600, Shandong, Peoples R China
5.Shandong Acad Agr Sci, Inst Qual Standard & Testing Technol Agroprod, Jinan 250000, Shandong, Peoples R China
关键词： Electrochemical immunosensor; Capsaicinoids; Thionine and Prussian blue-gold nanocomposites; Serum
期刊名称：JOURNAL OF SOLID STATE ELECTROCHEMISTRY （ 影响因子：2.647； 五年影响因子：2.53 ）
ISSN： 1432-8488
年卷期：
页码：
收录情况： SCI
摘要： In this paper, a dual-signal amplified electrochemical immunosensor for quantitative detection of capsaicinoids in serum was reported, which could be used to study the pharmacological properties of capsaicinoids. To improve the sensitivity of the immunosensor, the glassy carbon electrode was modified with Prussian blue-gold nanocomposites (PB-AuNPs) and thionine (TH). PB-AuNPs provided better electrical conductivity. At the same time, TH enhanced the stability of electron transfer, leading to superior electrochemical performance. Meanwhile, an excellent anti-capsaicinoids antibody (Ab) was used to ensure the sensitivity and specificity of the electrochemical immunosensor. The prepared immunosensor was tested using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under the optimal conditions, the proposed immunosensor displayed a wide linear range from 0.01 to 1000 ng/mL with a detection limit of 0.01 ng/mL and showed good recoveries (85.12-104.25%) and high stability. Additionally, the immunosensor was successfully applied to monitor capsaicinoids in real serum samples.
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