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Lentinan protects Caenorhabditis elegans against fluopyram-induced toxicity through DAF-16 and SKN-1 pathways

文献类型： 外文期刊

作者： Liu, Huimin 1 ; Liu, Bingjie 1 ; Zhang, Siqi 3 ; Fan, Miao 1 ; Ji, Xiaoxue 1 ; Zhang, Shouan 4 ; Wang, Zhongtang 2 ; Qiao, Kang 1 ;

作者机构： 1.Shandong Agr Univ, Coll Plant Protect, Key Lab Pesticide Toxicol & Applicat Tech, Tai An 271018, Shandong, Peoples R China

2.Shandong Inst Pomol, Taian City 271000, Shandong, Peoples R China

3.Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Shandong, Peoples R China

4.Univ Florida, IFAS, Trop Res & Educ Ctr, Dept Plant Pathol, Homestead, FL 33031 USA

关键词： Fluopyram; Lentinan; Caenorhabditis elegans; Toxicity; Oxidative damage

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2023 年 265 卷

页码：

收录情况： SCI

摘要： Fluopyram, a SDH inhibitor fungicide, is widely used in agriculture to control fungi and nematodes. However, fluopyram has been proved toxic that caused damage to organs through oxidative stress. The development of natural extracts that can reduce oxidative damage is a promising method. Lentinan is isolated from Lentinus edodes and has been verified its antioxidant activity. In this study, Caenorhabditis elegans was used to evaluate the protective effects of lentinan against fluopyram-induced toxicity and the possible mechanisms. Results showed that lentinan pretreatment notably increased the survival rate of N2 nematodes by 15.0 % and extended the lifespan by 91.5 %, compared with the fluopyram treatment. Lentinan pretreatment reverted the inhibition of the locomotion and reproduction of C. elegans under the fluopyram stress. In addition, lentinan pretreatment significantly decreased the contents of ROS and MDA in N2 nematodes. Moreover, pretreated with lentinan significantly recovered the decreased activities of CAT, SOD, GST and SDH induced by fluopyram. Lentinan pretreatment enhanced the mRNA levels of daf-16 and skn-1 and their downstream genes in the nematodes compared with the fluopyram group. In daf-16 and skn-1 mutants, the lifespan, ROS and related genes expression were not significantly changed in lentinan pretreatment. Pretreated with lentinan significantly enhanced the fluorescence intensity of SOD-3::GFP and GST-4::GFP, and promoted the nuclear translocation of DAF-16 and SKN-1 under the fluopyram stress. In summary, these findings indicated that lentinan protected C. elegans from fluopyram-induced toxicity via DAF-16 and SKN-1.

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