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Inhibitory effect of lactoferrin against gray mould on tomato plants caused by Botrytis cinerea and possible mechanisms of action

文献类型： 外文期刊

作者： Wang, Jie 1 ; Xia, Xiao-Ming 1 ; Wang, Hong-Yan 2 ; Li, Peng-Peng 1 ; Wang, Kai-Yun 1 ;

作者机构： 1.Shandong Agr Univ, Dept Plant Protect, Tai An 271018, Shandong, Peoples R China

2.Shandong Acad Agr Sci, Cotton Res Ctr, Jinan 250100, Shandong, Peoples R China

关键词： Lactoferrin; Botlytis cinerea; Membrane integrity; Cellular leakage; Methane dicarboxylic aldehyde

期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.277； 五年影响因子：5.388 ）

ISSN： 0168-1605

年卷期： 2013 年 161 卷 3 期

页码：

收录情况： SCI

摘要： Lactoferrin (LF), an 80 kDa iron binding glycoprotein, was effective in the control of gray mould caused by Botrytis cinerea on tomato. LF strongly inhibited spore germination and germ tube elongation of B. cinerea in vitro, especially at 100 mg/L. When viewed by fluorescence microscopy following staining with propidium iodide, the membrane integrity of B. cinerea conidia treated with LF (50 mg/L) decreased to 68% at 2 h. The leakage of carbohydrate and protein from mycelia increased as the dose of LF increased compared with the untreated controls. Furthermore, methane dicarboxylic aldehyde (MDA) content in B. cinerea spores exposed to LF increased markedly. The activities of superoxide dismutase and catalase in spores treated with LF were also 1.3, twice as high as those in the control at 6 h, whereas ATP content was 1.5 times lower. In planta assays demonstrated that LF exhibited significant curative effect (76.3%, 100 mg/L) against gray mould, compared with the preventive effect (52.6%, 100 mg/L). The mechanisms by which LF decreased gray mould decay of tomato plant may be directly related to the severe damage to the conidia plasma membrane and loss of cytoplasmic materials from the hyphae. These findings suggest that LF has potential as an antifungal agent to reduce the severity of gray mould of tomato plants. (C) 2012 Elsevier B.V. All rights reserved.

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