Catechin attenuates TNF-alpha induced inflammatory response via AMPK-SIRT1 pathway in 3T3-L1 adipocytes
文献类型： 外文期刊
作者： Cheng, An-Wei 1 ; Tan, Xin 1 ; Sun, Jin-Yue 1 ; Gu, Chun-Mei 4 ; Liu, Chao 1 ; Guo, Xu 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Jinan, Shandong, Peoples R China
2.Minist Agr, Key Lab Novel Food Resources Proc, Jinan, Shandong, Peoples R China
3.Key Lab Agroprod Proc Technol Shandong Prov, Jinan, Shandong, Peoples R China
4.Jilin Agr Univ, Coll Food Sci & Engn, Changchun, Jilin, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2019 年 14 卷 5 期
页码：
收录情况： SCI
摘要： Chronic inflammation is a fundamental symptom of many diseases. Catechin possesses anti-oxidant and anti-inflammatory properties. However, the mechanism of catechin to prevent inflammation in 3T3-L1 adipocytes caused by TNF-alpha remains unknown. Therefore, the effects of catechin on the gene expression of cytokines and the activation of cell signals in TNF-alpha induced 3T3-L1 adipocytes were investigated. The effects of catechin on adipogenesis and cell viability were detected by Oil Red O staining and CCK-8 assay, respectively. The genes expression of cytokines was determined by real-time RT-PCR. The expression of NF-kappa B, AMPK, FOXO3a and SIRT1 on translation level was determined by western blotting analysis. The results demonstrated that catechin significantly enhanced adipogenesis and cell viability. catechin inhibited the gene expression of pro-inflammatory cytokines including IL-1 alpha, IL-1 beta, IL-6, IL-12p35, and inflammatory enzymes including iNOS and COX2, but enhanced the gene expression of anti-inflammatory cytokines including IL-4 and IL-10. Catechin also inhibited the activation of NF-kappa B, AMPK, FOXO3a and SIRT1, but increased the phosphorylation level of the above factors. All these results indicated that as a potential therapeutic strategy catechin has the ability of attenuating inflammatory response triggered by TNF-alpha through signaling cascades involved in inflammation and cytokines.
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