文献类型： 外文期刊

作者： Zhang, Chen ² ; Li, Juan ³ ; Wang, Jing ⁴ ; Song, Xingling ² ; Zhang, Jianjun ² ; Wu, Shang ⁵ ; Hu, Chunlong ⁶ ; Gong, Zh ¹ ;

作者机构： 1.Shandong Acad Agr Sci, Key Lab Wastes Matrix Utilizat, Minist Agr, Inst Agr Resources & Environm, Jinan 250100, Shandong, Peoples R China

2.Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China

3.Chinese Acad Agr Sci, Beijing 100081, Peoples R China

4.Cent Hosp Taian, Tai An 271000, Shandong, Peoples R China

5.Shandong Agr Univ, Coll Food Sci, Tai An 271018, Shandong, Peoples R China

6.Shandong Agr Univ, Coll Forestry, Tai An 271018, Shandong, Peoples R China

关键词： Antihyperlipidaemic;Hepatoprotective;Antioxidant;Acidic and enzymatic hydrolysis exopolysaccharides;Pleurotus eryngii SI-04

期刊名称：BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE （ 影响因子：3.659； 五年影响因子：3.767 ）

ISSN： 1472-6882

年卷期： 2017 年 17 卷

页码：

收录情况： SCI

摘要： Background: Hyperlipidaemia is the major risk factor contributing to the development and progression of atherosclerosis, fatty liver and cerebrovascular disease. Pleurotus eryngii (P. eryngii) is rich in biologically active components, especially polysaccharides that exhibit various biological activities, including reducing blood lipids. In the present study, three novel polysaccharide types, including exopolysaccharides (EPS), enzymatic EPS (EEPS) and acidic EPS (AEPS) were isolated, and the hypolipidaemic and hepatoprotective effects were investigated to better understand possible hypolipidaemic mechanisms and their hepatoprotective effects. Methods: The EPS was hydrolysed by snailase (dissolved in 1% acetic acid, pH = 6) and H2SO4 (1 M) to obtain EEPS and AEPS, respectively. The in vitro antioxidant activities were measured by investigating the reducing power and the scavenging effects on radicals of hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion. The hyperlipidaemic mice were induced by perfusing a high-fat emulsion. In addition to the hepatic histopathology, the following biochemical analyses were performed to investigate the antioxidative effects, including the activities of alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT). Triacylglycerol (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA) and lipid peroxidation (LPO) levels were also measured in serum and liver homogenate. Results: Supplementation of EPS, EEPS and AEPS could significantly improve blood lipid levels (TC, TG, HDL-C, and LDL-C), hepatic lipid levels (TC and TG), hepatic enzyme activities (ALP, ALT, and AST) and antioxidant status (GSH-Px, SOD, T-AOC, MDA, and LPO). In addition, histopathological observations indicated that these polysaccharides had potential effects in attenuating hepatocyte damage. Conclusion: These results demonstrated that both EPS and its hydrolysates EEPS and AEPS might effectively reduce serum lipid levels and protect against high-fat diet-induced hyperlipidaemia, indicating that they could be used as functional foods and natural hepatoprotectants.