Enhancing the Sensitivity of the Bio-barcode Immunoassay for Triazophos Detection Based on Nanoparticles and Droplet Digital Polymerase Chain Reaction
文献类型： 外文期刊
作者： Cui, Xueyan 1 ; Jin, Maojun 1 ; Zhang, Chan 1 ; Du, Pengfei 2 ; Chen, Ge 1 ; Qin, Guoxin 3 ; Jiang, Zejun 1 ; Zhang, Yudan 1 ; Li, Mingjie 1 ; Liao, Yun 1 ; Wang, Yuanshang 1 ; Cao, Zhen 1 ; Yang, Feiyan 3 ; Abd El-Aty, A. M. 4 ; Wang, Jing 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Jinan 250100, Shandong, Peoples R China
3.Guangxi Acad Agr Sci, Agroprod Qual Safety & Testing Technol Res Inst, Nanning 530007, Guangxi, Peoples R China
4.Cairo Univ, Dept Pharmacol, Fac Vet Med, Giza 12211, Egypt
5.Ataturk Univ, Fac Med, Dept Med Pharmacol, TR-25240 Erzurum, Turkey
关键词： gold nanoparticles; bio-barcode; magnetic nanoparticles; ddPCR; triazophos
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2019 年 67 卷 46 期
页码：
收录情况： SCI
摘要： An ultrasensitive bio-barcode competitive immunoassay method based on droplet digital polymerase chain reaction (ddPCR) was developed for the determination of triazophos. Gold nanoparticles (AuNPs) were coated with monoclonal antibodies (mAbs) and complementary double-stranded DNA (dsDNA), which included bio-barcode DNA and thiol-capped DNA. Magnetic nanoparticle (MNP) probes were constructed by modifying the MNPs with ovalbumin-hapten conjugates (OVA-hapten). The target pesticide and OVA-hapten on the surface of the MNP probes competed with the AuNP probes simultaneously, and then the bio-barcode DNA was released for quantification by ddPCR. The concentration of released DNA was inversely proportional to the concentration of pesticide to be tested. Under the optimum conditions, the competitive immunoassay exhibited a wide linear range of 0.01-20 ng/mL and a low detection limit of 0.002 ng/mL. Spike recovery tests were carried out using apple, rice, cabbage, and cucumber samples to verify the feasibility of the method. The recovery and relative standard deviations (RSDs) of the technique ranged from 76.9 to 94.4% and from 10.8 to 19.9%, respectively. To further validate the results, a linear correlation analysis was performed between the proposed method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Consequently, the bio-barcode immunoassay based on nanoparticles and ddPCR, an ultrasensitive method, showed great potential for the determination of target pesticides in real samples.
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