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Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis

文献类型： 外文期刊

作者： Yang, Maohua 1 ; Zhang, Xiang 2 ;

作者机构： 1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China

2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, 202 North Ind Rd, Jinan 250100, Peoples R China

关键词： Pyruvate;Escherichia coli;Transcription analysis;Oxidation-Reduction;Flagellar assembly

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN：

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收录情况： SCI

摘要： To obtain strain YP211 with a high tendency for accumulating pyruvate, central metabolic pathways were modified in Escherichia coli MG1655. Specifically, seven genes (ldhA, pflB, pta-ackA, poxB, ppc, frdBC) were knocked out sequentially and full pyruvate dehydrogenase was retained. In batch fermentation with M9 medium, pyruvate yield and production rate reached 0.63 g/g glucose and 1.89 g/(1 h), respectively. Meanwhile, the production of acetate, succinate, and other carboxylates was effectively controlled. To understand the physiological observations, we further completed genome-wide transcription analysis of wild-type and YP211. As the acetic acid pathways were blocked, the pathways of convertion of pyruvate to phosphoenol pyruvate and acetyl CoA were enhanced. The transcription of pck, as an alternative gene for ppc, was increased by 2.6 times. So even if gene ppc was inactivated, the tricarboxylic acid pathway was still enhanced in YP211. In order to balance intracellular NADH/NAD(+), oxidative phosphorylation and flagellar assembly system were also up-regulated significantly.

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