Interactive mechanism between avian infectious bronchitis S1 protein T cell peptide and avian MHC I molecule
文献类型： 外文期刊
作者： Zhu, Feng-zhu 1 ; Luci, Mei; Huang, Qing-hua 1 ; Huang, Yan-yan 1 ; Yang, Shao-hua 1 ; Cui, Yan-shun 2 ; Liu, Chang 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Jinan 2501001, Shandong, Peoples R China
2.Shandong Agr Univ, Coll Anim Sci, Tai An 271018, Shandong, Peoples R China
3.Shandong Agr Univ, Coll Anim Sci, Tai An 271018, Shandon
关键词： Homology modelling;Avian infectious bronchitis virus;CTL epitope
期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）
ISSN：
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收录情况： SCI
摘要： This study aims to construct a 3D structure of the avian major histocompatibility complex (MHC)-beta 2M complex through homology modelling technology, perform molecular docking of the predicted infectious bronchitis virus (IBV) S1 protein potential epitope peptide Sp6 (NQFYIKLT) and the avian MHC-beta 2M complex, and demonstrate the interactive mechanism between Sp6 and MHC using molecular dynamical simulations. The peptide Sp6 and the non-related peptide NP89-97 (PKKTGGPIY) were used to stimulate in vitro recombinant plasmid (pCAGGS-S1) avian splenic lymphocytes. Flow cytometric results show that CD8(+) T lymphocytes reproduce stimulated by the Sp6 and the nonrelated peptide proliferate by 34.8% and 2.6%, respectively. Meanwhile, fluorescent quantitative PCR results show that the secretion of IFN-gamma in avian splenic lymphocytes increases after Sp6 stimulation. These data suggest that Sp6 can induce the activated avian lymphocytes in vitro to produce CTL, which is the CTL epitope in IBV S1. (C) 2016 Elsevier B.V. All rights reserved.
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