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Preparation of anti-melamine antibody and development of an indirect chemiluminescent competitive ELISA for melamine detection in milk

文献类型： 外文期刊

作者： Li, Weihua 1 ; Meng, Meng 2 ; Lu, Xiao 3 ; Liu, Wei 1 ; Yin, Weiwei 1 ; Liu, Jinting 1 ; Xi, Rimo 2 ;

作者机构： 1.Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China

2.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China

3.Shandong Acad Agr Sci, Cent Lab, Jinan, Peoples R China

期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

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收录情况： SCI

摘要： An indirect chemiluminescent competitive ELISA (CL-ELISA) method was developed to detect residues of melamine in milk. A high-quality polyclonal antibody towards melamine cyanurate (MC) was prepared based on synthesis of a novel immunogen. The method is applicable over the range of 0.5-7.0 mu g.mL(-1) of MC, with an IC50 value of 1.7 mu g.mL(-1). The developed method was used in the detection of melamine residue in milk with the detection limit of 1 ng.mL(-1). There was no cross-reactivity with commonly used veterinary drugs. The CL-ELISA method developed provides an alternative to chromatography spectrometry for regulatory analysis of melamine in milk and could be promisingly used to improve the sensitivity of the available ELISA test kits

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