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Generation of Biotechnology-Derived Flavobacterium columnare Ghosts by PhiX174 Gene E-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp

文献类型： 外文期刊

作者： Zhu, Wenxing 1 ; Yang, Guiwen 1 ; Zhang, Yuyu 2 ; Yuan, Jinduo 1 ; An, Liguo 1 ;

作者机构： 1.Shandong Normal Univ, Coll Life Sci, Key Lab Anim Resistance Biol Shandong, Jinan 250014, Peoples R China

2.Shandong Acad Agr Sci, Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China

期刊名称：JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY （ 影响因子：3.169； 五年影响因子：3.132 ）

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收录情况： SCI

摘要： Flavobacterium columnare is a bacterial pathogen causing high mortality rates for many freshwater fish species. Fish vaccination with a safe and effective vaccine is a potential approach for prevention and control of fish disease. Here, in order to produce bacterial ghost vaccine, a specific Flavobacterium lysis plasmid pBV-E-cat was constructed by cloning PhiX174 lysis gene E and the cat gene with the promoter of F. columnare into the prokaryotic expression vector pBV220. The plasmid was successfully electroporated into the strain F. columnare G4cpN22 after curing of its endogenous plasmid. F. columnare G4cpN22 ghosts (FCGs) were generated for the first time by gene E-mediated lysis, and the vaccine potential of FCG was investigated in grass carp (Ctenopharyngodon idellus) by intraperitoneal route. Fish immunized with FCG showed significantly higher serum agglutination titers and bactericidal activity than fish immunized with FKC or PBS. Most importantly, after challenge with the parent strain G4, the relative percent survival (RPS) offish in FCG group (70.9%) was significantly higher than FKC group (41.9%). These results showed that FCG could confer immune protection against F. columnare infection. As a nonliving whole cell envelope preparation, FCG may provide an ideal alternative to pathogen-based vaccines against columnaris in aquaculture.

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