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Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle

文献类型： 外文期刊

作者： Hou, Qinlei 1 ; Huang, Jinming 1 ; Ju, Zhihua 1 ; Li, Qiuling 1 ; Li, Liming 1 ; Wang, Changfa 1 ; Sun, Tao 1 ; Wang, Lin 1 ;

作者机构： 1.Shandong Acad Agr Sci, Lab Mol Genet & Breeding, Ctr Dairy Cattle Res, Jinan 250131, Shandong, Peoples R China

2.Shandong Acad Ag

关键词： amino acids;bovine mastitis;cows;dairy cattle;dairy cows;disease resistance;exons;genes;genetic resistance;immune response;major histocompatibility complex;mammary gland diseases;mammary glands;mastitis;single nucleotide polymorphism;cattle;Bos;Bovidae;ruminants;Artiodactyla;ungulates;mammals;vertebrates;Chordata;animals;eukaryotes;histocompatibility complex;immunity reactions;immunological reactions;microRNA;resistance to disease

期刊名称：DNA AND CELL BIOLOGY （ 影响因子：3.311； 五年影响因子：3.557 ）

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收录情况： SCI

摘要： Major histocompatibility complex, class II, DQ alpha 2, also named BOLA-DQA2, belongs to the Bovine Leukocyte Antigen (BOLA) class II genes which are involved in the immune response. To explore the variability of the BOLA-DQA2 gene and resistance to mastitis in cows, the splice variants (SV), targeted microRNAs (miRNAs), and single nucleotide polymorphisms (SNPs) were identified in this study. A new SV (BOLA-DQA2-SV1) lacking part of exon 3 (195 bp) and two 3'-untranslated regions (UTR) (52 bp + 167 bp) of the BOLA-DQA2 gene was found in the healthy and mastitis-infected mammary gland tissues. Four of 13 new SNPs and multiple nucleotide polymorphisms resulted in amino acid changes in the protein and SNP (c. + 1283 C > T) may affect the binding to the seed sequence of bta-miR-2318. Further, we detected the relative expressions of two BOLA-DQA2 transcripts and five candidated microRNAs binding to the 3'-UTR of two transcripts in the mammary gland tissues in dairy cattle by using the quantitative real-time polymerase chain reaction. The result showed that expression of the BOLA-DQA2-SV1 mRNA was significantly upregulated 2.67-fold (p < 0.05) in mastitis-infected mammary tissues (n = 5) compared with the healthy mammary gland mammary tissues (n = 5). Except for bta-miR-1777a, miRNA expression (bta-miR-296, miR-2430, and miR-671) was upregulated 1.75 to 2.59-fold (p < 0.05), whereas miR-2318 was downregulated in the mastitis cows. Our findings reveal that BOLA-DQA2-SV1 may play an important role in the mastitis resistance in dairy cattle. Whether the SNPs affect the structure of the BOLA-DQA2 gene or association with mastitis resistance is unknown and warrants further investigation.

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