文献类型: 外文期刊
作者: Liu, Xu 1 ; Sun, Xu 1 ; Wang, Wenying 1 ; Ding, Hanfeng 1 ; Liu, Wei 1 ; Li, Guangxian 2 ; Jiang, Mingsong 2 ; Zhu, Chang 1 ;
作者机构: 1.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan 250100, Peoples R China
2.Shandong Rice Res Inst, Jinan 250100, Peoples R China
3.Shandong Agr Univ, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
关键词: Pa-6;Physical mapping;Purple apiculus;Rice;Single-segment substitution line (SSSL)
期刊名称:JOURNAL OF PLANT BIOLOGY ( 影响因子:2.434; 五年影响因子:2.455 )
ISSN:
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收录情况: SCI
摘要: Purple apiculus is one of the important agronomic traits of rice. Single-segment substitution line (SSSL) W23-07-6-02-14 in the genetic background of an elite rice variety Huajingxian74 (HJX74) with the substituted interval of RM225-RM217-RM253 on the chromosome 6 was found to have purple apiculus (Pa). To map the gene governing Pa, W23-07-6-02-14 was crossed with the recipient HJX74 to develop an F-2 secondary segregation population. The ratio of purple apiculus to green apiculus showed a good fit to 3:1 ratio, indicating that Pa was controlled by a major dominant gene. The gene locus for Pa was tentatively designated as Pa-6. Using 430 individuals from the F-2 segregation population, the Pa-6 locus was mapped between two SSR markers RM19556 and RM19561 with genetic distances of 0.2 and 0.3 cM, respectively. For fine mapping of the Pa-6 gene, a large F-2:3 segregation population of 3890 individuals was developed from F-2 heterzygous plants in the RM19556-RM19561 region. Recombinant analyses further mapped the Pa-6 gene locus to an interval of 41.7-kb bounded L02 and RM19561. Sequence analysis of this 41.7-kb region revealed that it contains eleven open reading frames (ORFs), of which, ORF5 is classified as the one that is associated with the C (chromogen for anthocyanin) gene, it was presumed to be the candidate gene for Pa. This result provided a foundation of map-based cloning and function analysis of the Pa-6 gene.
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