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Characterization of the pyruvate kinase gene family in soybean and identification of a putative salt responsive gene GmPK21

文献类型： 外文期刊

作者： Liu, Wei 1 ; Wang, Yubin 1 ; Zhang, Yanwei 1 ; Li, Wei 1 ; Wang, Caijie 1 ; Xu, Ran 1 ; Dai, Haiying 1 ; Zhang, Lifeng 1 ;

作者机构： 1.Shandong Acad Agr Sci, Crop Res Inst, 202 Gongye North Rd, Jinan 250100, Shandong, Peoples R China

2.Shandong Engn Lab Featured Crops, 202 Gongye North Rd, Jinan 250100, Shandong, Peoples R China

关键词： Soybean; Pyruvate Kinase gene family; Genome identification; Salt stress Overexpression

期刊名称：BMC GENOMICS （ 影响因子：4.4； 五年影响因子：4.7 ）

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： Background As a key regulatory enzyme in the glycolysis pathway, pyruvate kinase (PK) plays crucial roles in multiple physiological processes during plant growth and is also involved in the abiotic stress response. However, little information is known about PKs in soybean. Results In this study, we identified 27 PK family genes against the genome of soybean cultivar Zhonghuang13. They were classified into 2 subfamilies including PKc and PKp. 22 segmental duplicated gene pairs and 1 tandem duplicated gene pair were identified and all of them experienced a strong purifying selective pressure during evolution. Furthermore, the abiotic stresses (especially salt stress) and hormone responsive cis-elements were present in the promoters of GmPK genes, suggesting their potential roles in abiotic stress tolerance. By performing the qRT-PCR, 6 GmPK genes that continuously respond to both NaCl and ABA were identified. Subsequently, GmPK21, which represented the most significant change under NaCl treatment was chosen for further study. Its encoded protein GmPK21 was localized in the cytoplasm and plasma membrane. The transgenic Arabidopsis overexpressing GmPK21 exhibited weakened salinity tolerance. Conclusions This study provides genomic information of soybean PK genes and a molecular basis for mining salt tolerance function of PKs in the future.

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