A loop-mediated isothermal amplification method for rapid detection of the multidrug-resistance gene cfr
文献类型： 外文期刊
作者： Qi, Jing 1 ; Du, Yijun 1 ; Zhu, Rongsheng 1 ; Zhu, Xiaoling 1 ; Bai, Hua 1 ; Hu, Ming 1 ; Luo, Yanbo 1 ; Hu, Xinxin 1 ; Wu, 1 ;
作者机构： 1.Shandong Acad Agr Sci, Shandong Key Lab Anim Dis Control & Breeding, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China
2.Shandong Acad Agr Sci, S
关键词： Loop-mediated isothermal amplification (LAMP);Rapid detection;Sensitivity;The multidrug-resistance gene cfr
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
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收录情况： SCI
摘要： We developed and evaluated the specificity and sensitivity of a loop-mediated isothermal amplification (LAMP) method for rapid detection of the multidrug-resistance gene . cfr. A pair of outer primers and a pair of inner primers and one loop primer were specially designed for recognizing seven distinct sequences on the target . cfr gene. The amplification reaction was performed within only 35. min under isothermal conditions at 63. °C in a regular water bath with visual measurement. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of 1. pg per tube of chicken . Staphylococcus sciuri genomic DNA. The detection rate of . cfr gene for 50 Staphylococcus clinical strains by LAMP assays was 16% and appeared 100% consistence with the result by PCR method. The LAMP method reported here demonstrated a potential and valuable means for detection of the multidrug-resistance gene . cfr: easy, rapid, visual, specific, accurate and sensitive, especially useful for on-the-spot investigation.
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