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Six novel single-nucleotide polymorphisms in SPAG11 gene and their association with sperm quality traits in Chinese Holstein bulls

文献类型： 外文期刊

作者： Liu, Xinyan 1 ; Ju, Zhihua 1 ; Wang, Lingling 1 ; Zhang, Yan 1 ; Huang, Jingmin 1 ; Li, Qiuling 1 ; Li, Jianbin 1 ; Zhong 1 ;

作者机构： 1.Shandong Acad Agr Sci, Lab Dairy Cattle Reprod, Dairy Cattle Res Ctr, Jinan 250100, Peoples R China

2.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China

3.Shandong Normal Univ, Coll Life Sci, Jinan 2500

关键词： SPAG11 gene;SNPs;Combined genotype;Sperm quality trait;Chinese Holstein bull

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

ISSN：

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收录情况： SCI

摘要： Sperm-associated antigen 11 (SPAG11) is predominant in the male reproductive tract. Similar to P-defensin, aside from its antibacterial activity, SPAG11 also has an important role in male reproductive function. In the present study, the association of bovine SPAG11 gene polymorphism with sperm quality traits was examined, including ejaculate volume, sperm concentration, fresh sperm motility, post-thaw cryopreserved sperm motility, and deformity rate of bull semen. Six novel single nucleotide polymorphisms (SNPs) of the SPGA11 gene were investigated in 426 normal mature Chinese Holstein bulls using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), PCR-restriction fragment length polymorphism (PCR-RFLP), created restriction site-PCR (CRS-PCR), and DNA sequencing methods. Linkage disequilibrium analysis showed that g.1306G>A and g.1454G>A (SNP-1), and g.16904G>T, g.16974C>T, and g.17000A>G (SNP-2) are completely linked, respectively. Correlation analysis showed the SNP-2 marker had a marked effect on fresh sperm motility and sperm concentration (P < 0.05). SNP-3 g.22696T>C had a marked effect on post-thaw cryopreserved sperm motility (P < 0.05) and deformity rate (P < 0.01). However, the presence of SNP-1 was not correlated with the sperm production traits (P > 0.05). Furthermore, association analyses of the 8 haplotypes constructed from the 17 combined haplotypes and reproductive traits showed that the bulls with the combined haplotype H5H6 (GGT/TTC) have the highest ejaculate volumes and the bulls with combined haplotypes H1H1 (AAT/TTT) and H1H6 (AGT/TTC) had the highest fresh and post-thaw sperm motilities, respectively. These results indicate that new molecular markers associated with sperm quality traits can be used in marker-assisted selection in bull breeding programs. (C) 2011 Elsevier B.V. All rights reserved.

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