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In vitro maturation and artificial activation of donkey oocytes

文献类型： 外文期刊

作者： Zhao, Gaoping 2 ; Wu, Kaifeng 1 ; Cui, Liang 1 ; Zhao, Lixia 1 ; Liu, Yiyi 1 ; Tan, Xiuwen 3 ; Zhou, Huanmin 1 ;

作者机构： 1.Inner Mongolia Agr Univ, Coll Life Sci, Hohhot 010018, Peoples R China

2.Inner Mongolia Agr Univ, Coll Anim Sci & Vet Med, Hohhot 010018, Peoples R China

3.Shandong Acad Agr Sci, Acad Anim Husb & Vet Med, Jinan 250000, Peoples R China

关键词： transferrin;insulin: 9004-10-8;selenium: 7782-49-2;fetal calf serum;L-cysteine: 52-90-4;taurine: 107-35-7;FSH: 9002-68-0;L-glutamine: 56-85-9;sodium pyruvate: 113-24-6;insulin-like growth factor-1: IGF-I;67763-96-6;gentamycin: 1403-66-3;in vitro maturation;parthenogenetic activation;embryo development;maturation rate;artificial activation

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Three media were evaluated for their ability to support in vitro maturation of donkey (Equus asinus) oocytes and their development after parthenogenetic activation. The basal medium for Medium 1 (M1) and Medium 2 (M2) was M199 and DMEM/F12 respectively, whereas, Medium 3 (M3) consisted of equal parts (v/v) of M199 and DMEM/F12. All three media were supplemented with 10% (v/v) fetal calf serum, 0.01 units/mL porcine FSH, 0.01 units/mL equine LH, 200 ng/mL insulin-like growth factor 1(IGF-1), 10 mu l/mL insulin-transferrin-selenium (ITS), 0.1 mg/mL taurine, 0.1 mg/mL L-cysteine, 0.05 mg/mL L-glutamine, 0.11 mg/mL sodium pyruvate, and 25 mg/mL gentamycin. There were no significant differences among the three maturation media for oocyte maturation. Maturation rate of donkey oocytes in M1 was 53% for compact (Cp) cumulus-oocyte complexes and 75% for expanded (Ex) cumulus-oocyte complexes; in M2 these were 55 and 77%, respectively; and in M3, 58 and 75%. The percentage of cleaved parthenotes and 4- or 8-cell embryos were not significantly different for oocytes matured in the various media (61 and 24% for M1; 66 and 32% for M2; and 67 and 33% for M3). Oocytes matured in M3 tended to yield a higher rate of advanced embryo development (morula) than oocytes matured in M1 (22 vs 9%; P = 0.07). In conclusion, donkey oocytes were matured and parthenogenetically activated in vitro, using methods similar to those used in the horse

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