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Single-step chromatography for simultaneous purification of C-phycocyanin and allophycocyanin with high purity and recovery from Spirulina (Arthrospira) platensis

文献类型： 外文期刊

作者： Yan, Shi-Gan 1 ; Zhu, Li-Ping 2 ; Su, Hai-Nan 1 ; Zhang, Xi-Ying 1 ; Chen, Xiu-Lan 1 ; Zhou, Bai-Cheng 1 ; Zhang, Yu-Z 1 ;

作者机构： 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China

2.Shandong Inst Light Ind, Sch Food & Bioengn, Jinan 250353, Peoples R China

3.Shandong Acad Agr Sci, Jinan 250100, Peoples R China

关键词： C-phycocyanin;Allophycocyanin;Anion exchange chromatography;pH gradient;Purification

期刊名称：JOURNAL OF APPLIED PHYCOLOGY （ 影响因子：3.215； 五年影响因子：3.612 ）

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收录情况： SCI

摘要： The cyanobacterium Spirulina (Arthrospira) platensis is a good source of phycobiliprotein purification. C-phycocyanin (C-PC) is the major phycobiliprotein, while allophycocyanin (APC) is less abundant in S. platensis. Previously reported methods for C-PC purification are only able to offer either high purity or high efficiency. This paper describes one-step anion exchange chromatography method with continuous pH gradient elution for simultaneous purification of C-PC and APC with high purity and highrecovery. Crude C-PC and APC were extracted and concentrated by ammonium sulfate fractionation at saturation of 25% and 60%, then purified on a DEAE-Sepharose Fast Flow chromatography column with continuous pH gradient elution from pH 5.0 to 3.6. After this single-step chromatography, C-PC and APC with high purity and recovery were simultaneously obtained. The purity ratios of C-PC and APC reached 5.59 (A620/A280) and 5.19 (A650/A280), respectively. Their purity was further demonstrated by electrophoresis and fluorescence emission spectroscopy. Moreover, the total recovery yield of pure C-PC and APC were 67.04% and 80.0%, representing 111.83 and 29.28 mg g~(-1) lyophilized weight, respectively. The obtained C-PC and APC remained stable over a pH rangeof 4–9. This purification method for high purity and recovery of C-PC and APC proved to be fairly efficient compared with previously reported methods.

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