您好，欢迎访问山东省农业科学院文献资源数据库平台

Identification and Characterization of Three Chitinases with Potential in Direct Conversion of Crystalline Chitin into N,N '-diacetylchitobiose

文献类型： 外文期刊

作者： Ren, Xue-Bing 1 ; Dang, Yan-Ru 1 ; Liu, Sha-Sha 1 ; Huang, Ke-Xuan 1 ; Qin, Qi-Long 1 ; Chen, Xiu-Lan 1 ; Zhang, Yu-Zhong 1 ; Wang, Yan-Jun 1 ; Li, Ping-Yi 1 ;

作者机构： 1.Shandong Univ, Inst Marine Sci & Technol, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China

2.Ocean Univ China, Coll Marine Life Sci, Frontiers Sci Ctr Deep Ocean Multispheres & Earth, Qingdao 266003, Peoples R China

3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China

4.Shandong Acad Agr Sci, Inst Wetland Agr & Ecol, Jinan 250100, Peoples R China

关键词： chitinases; crystalline chitin; chitooligosaccharides; N,N '-diacetylchitobiose; Pseudoalteromonas

期刊名称：MARINE DRUGS （ 影响因子：5.118； 五年影响因子：5.951 ）

ISSN：

年卷期： 2022 年 20 卷 3 期

页码：

收录情况： SCI

摘要： Chitooligosaccharides (COSs) have been widely used in agriculture, medicine, cosmetics, and foods, which are commonly prepared from chitin with chitinases. So far, while most COSs are prepared from colloidal chitin, chitinases used in preparing COSs directly from natural crystalline chitin are less reported. Here, we characterize three chitinases, which were identified from the marine bacterium Pseudoalteromonas flavipulchra DSM 14401(T), with an ability to degrade crystalline chitin into (GlcNAc)(2) (N,N'-diacetylchitobiose). Strain DSM 14401 can degrade the crystalline alpha-chitin in the medium to provide nutrients for growth. Genome and secretome analyses indicate that this strain secretes six chitinolytic enzymes, among which chitinases Chia4287, Chib0431, and Chib0434 have higher abundance than the others, suggesting their importance in crystalline alpha-chitin degradation. These three chitinases were heterologously expressed, purified, and characterized. They are all active on crystalline alpha-chitin, with temperature optima of 45-50 degrees C and pH optima of 7.0-7.5. They are all stable at 40 degrees C and in the pH range of 5.0-11.0. Moreover, they all have excellent salt tolerance, retaining more than 92% activity after incubation in 5 M NaCl for 10 h at 4 degrees C. When acting on crystalline alpha-chitin, the main products of the three chitinases are all (GlcNAc)(2), which suggests that chitinases Chia4287, Chib0431, and Chib0434 likely have potential in direct conversion of crystalline chitin into (GlcNAc)(2).

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台