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Tripeptides From Casein Are Signal Molecules to Induce the Expression of the Extracellular Protease MCP-01 Gene in Marine Bacterium Pseudoalteromonas sp. SM9913

文献类型： 外文期刊

作者： Chen, Xiu-Lan 1 ; Yang, Jin-Yu 1 ; Zheng, Xiao-Yu 1 ; Sheng, Qi 1 ; Wang, Lei 1 ; Zhang, Yu-Zhong 1 ; Qin, Qi-Long 1 ; Zh 1 ;

作者机构： 1.Shandong Univ, Marine Biotechnol Res Ctr, State Key Lab Microbial Technol, Qingdao, Shandong, Peoples R China

2.Minist Agr & Rural Affairs, Inst Agrofood Sci & Technol, Shandong Acad Agr Sci, Key Lab Agroprod Proc Technol Shandong Prov,Key L, Jinan, Shandong, Peoples R China

3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China

4.Ocean Univ China, Coll Marine Life Sci, Qingdao, Shandong, Peoples R China

关键词： signal molecule; extracellular protease; induce; marine bacteria; tripeptide

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： Microbial extracellular proteases play crucial roles in marine protein degradation and nitrogen recycling. Although a large number of marine bacteria are found to produce extracellular proteases, it is still unknown how marine bacteria respond to environmental proteins to activate the expression of genes encoding extracellular proteases. The inducing signal molecule for marine bacterial extracellular proteases has never been identified. In this study, we identified tripeptides as the inducing signal molecules for the extracellular protease MCP-01 of the deep-sea bacterium Pseudoalteromonas sp. SM9913. We found that casein, but not casamino acids, can induce the gene expression and synthesis of MCP-01, suggesting that peptides rather than amino acids derived from casein induce the gene expression and synthesis of MCP-01 in SM9913. Then, casein was hydrolyzed by SM9913 extracellular proteases, and the peptides with inducing effect were isolated and characterized. Finally, four tripeptides, SPP, RYP, RQF and FRQ, were shown to have significant inducing effect on the expression of MCP-01 gene, indicating that they are likely the inducing signal molecules for the expression of protease MCP-01 gene in SM9913. This study sheds light on the induction mechanism for the gene expression and biosynthesis of marine microbial extracellular proteases, which is helpful in better understanding the adaptation of bacteria to deep-sea sedimental environment.

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