Magnetic Separation Immunosensor Based on Enzyme-linked Gold Nanocomposite Probe for Detection of Ractopamine
文献类型： 外文期刊
作者： HE, Feng 1 ; WANG, Hai-Jie 2 ; LI, Teng-Fei 1 ; DU, Peng-Fei 2 ; WANG, Wei-Ting 2 ; WANG, Shou-Jing 2 ; LIU, Yao-Bo 2 ; MA, Yan-Li 2 ; HU, Peng 2 ; TAN, Tian-Yu 1 ; HAN, Qing 1 ;
作者机构： 1.Hebei Univ Engn, Sch Life Sci & Food Engn, Handan 056038, Peoples R China
2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Key Lab Novel Food Resources Proc, Shandong Prov Key Lab Agroprod Proc Technol,Minist, Jinan 250100, Peoples R China
3.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China
关键词： Ractopamine; Gold nanoparticles; Magnetic microbeads; Horseradish peroxidase
期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.2； 五年影响因子：1.0 ）
ISSN： 0253-3820
年卷期： 2023 年 51 卷 1 期
页码：
收录情况： SCI
摘要： A magnetic separation competitive immunosensor based on novel enzyme-linked gold nanocomposite probes (E-GNPs) was constructed for detection of ractopamine ( RAC). Through the specific interaction between streptavidin and biotin, horseradish peroxidase ( HRP) was labeled on the antibody that was modified on the surface of gold nanoparticles (AuNPs) by electrostatic assembly method to obtain E-GNPs. Ovalbumin (OVA )-RAC hapten coated magnetic beads ( MBs ) was introduced for a competitive reaction, and quantitative analysis of the target was achieved by a color reaction. With the change of the concentration of target molecule RAC, the color of the enzyme-catalyzed substrate changed, and the concentration of RAC had a linear relationship with the color signal intensity. The UV-vis absorption spectroscopy characterization results showed that one E-GNP could carry 11 HRP molecules, which made the immunosensor highly sensitive. The prepared sensor was successfully used for detection of RAC with a detection limit of 1.75 pg/ mL, showing 10 times higher sensitivity than that of traditional ELISA method. Cross-reaction experiments showed that the sensor had good selectivity for RAC, and could be applied to real samples such as pork, beef and mutton, with spiked recoveries ranging from 88. 3% to 103. 4%. This analytical method provided a new idea for rapid screening of RAC in animal-derived foods.
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