文献类型： 外文期刊
作者： Yang, Chunhong 1 ; Wang, Jinpeng 1 ; Liu, Juan 1 ; Sun, Yan 1 ; Guo, Yijun 1 ; Jiang, Qiang 1 ; Ju, Zhihua 1 ; Gao, Qican 1 ; Wang, Xiuge 1 ; Huang, Jinming 1 ; Wang, Changfa 1 ;
作者机构： 1.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan 250131, Shandong, Peoples R China
2.Shandong Normal Univ, Coll Life Sci, Jinan, Shandong, Peoples R China
3.Shandon
关键词： Bovine; ARID4A; SNP; Haplotype; Semen quality
期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）
ISSN： 0378-4320
年卷期： 2018 年 197 卷
页码：
收录情况： SCI
摘要： The AT-rich interaction domain 4 A (ARID4A) has an important role in regulating Sertoli cell function and male fertility. Its molecular mechanisms, however, remain largely unknown. In this study, two single nucleotide polymorphisms (SNPs) (g.53 G > T, ss 1966531596, and g.826 G > A, rs 210809648) were identified in the promoter region of ARID4A in 215 Chinese Holstein bulls using polymerase chain reaction (PCR)-restriction fragment length polymorphism and created restriction site-PCR. Results revealed that bulls with g.53 G > T-GG and g.826 G > A-G G genotype exhibited higher sperm deformity rate than those with g.53 G > T-TT and g.826 G > A-AA genotype (P < 0.01). Furthermore, three haplotypes (H1 (GG), H3 (TG), H4 (TA)) and six haplotype combinations (H1H1, H1H3, H1H4, H3H3, H3H4, H4H4) were obtained. The bulls with H4H4 exhibited lower sperm deformity rate than those with H1H1 and H1H3 (P < 0.05). In addition, results of bioinformatics analysis revealed that ARID4A has two promoters and that two SNPs of ARID4A are located in transcription factor binding sites. Compared with g.53 G > T-G and g.826 G > A-G allele, there was a greater fluorescence intensity in g.53 G > T-T and g.826 G > A-A allele by transient transfection in MLTC-1 cells and the luciferase report assay. qRT-PCR indicated the ARID4A expression was greater in bull spermatozoa with H4H4 haplotype combination than those with H1H1 haplotype combination (P < 0.05). Results of the present study indicate that g.53 G > T and g.826 G > A are functional mutations that are involved in regulation of ARID4A gene expression by affecting promoter activity and thus semen quality of Chinese Holstein bulls.
- 相关文献
作者其他论文 更多>>
-
CFAP58 is involved in the sperm head shaping and flagellogenesis of cattle and mice
作者：Wei, Xiaochao;Wang, Xiuge;Yang, Chunhong;Gao, Yaping;Zhang, Yaran;Xiao, Yao;Ju, Zhihua;Jiang, Qiang;Wang, Jinpeng;Liu, Wenhao;Li, Yanqin;Gao, Yundong;Huang, Jinming;Wei, Xiaochao;Wang, Xiuge;Yang, Chunhong;Gao, Yaping;Zhang, Yaran;Xiao, Yao;Ju, Zhihua;Jiang, Qiang;Wang, Jinpeng;Liu, Wenhao;Li, Yanqin;Gao, Yundong;Huang, Jinming;Gao, Yundong;Huang, Jinming
关键词：CFAP58; Genetic variation; Spermatogenesis; Manchette; IMT/IFT
-
Characterizing the hindgut microbiome in healthy and ketotic cows
作者：Ju, Zhihua;Li, Rongling;Liu, Wenhao;Jiang, Qiang;Wang, Jinpeng;Zhang, Yaran;Wang, Xiuge;Xiao, Yao;Yang, Chunhong;Wei, Xiaochao;Gao, Yaping;Huang, Jinming;Ju, Zhihua;Li, Rongling;Liu, Wenhao;Wang, Jinpeng;Zhang, Yaran;Wang, Xiuge;Xiao, Yao;Huang, Jinming;Ju, Zhihua;Huang, Jinming
关键词：Cow ketosis; beta-hydroxybutyrate; 16S sequencing; hindgut fecal microbiota
-
Integrated physiological, transcriptomics and metabolomics analysis revealed the molecular mechanism of Bupleurum chinense seedlings to drought stress
作者：Feng, Xiaohan;Sun, Yan;Fan, Ya;Gao, Demin;Zhang, Quanfang;Bu, Xun
关键词：
-
Evolutionary adaptations generally reverse phenotypic plasticity to restore ancestral phenotypes during new environment adaptation in cattle
作者：Jiang, Qiang;Cai, Yafei;Jiang, Qiang;Huang, Jinming;Zhu, Li;Zeng, Hao;Basang, Zhuzha;Suolang, Quji
关键词：cattle; environment adaptation; gene expression; phenotypic plasticity
-
Effect of Lactobacillus salivarius SNK-6 on egg quality, intestinal morphology, and cecal microbial community of laying hens
作者：Liu, Wei;Liu, Jie;Li, Dapeng;Han, Haixia;Sun, Yan;Lei, Qiuxia;Wang, Jie;Zhou, Yan;Cao, Dingguo;Li, Huimin;Li, Fuwei;Liu, Wei;Liu, Jie;Li, Dapeng;Han, Haixia;Sun, Yan;Lei, Qiuxia;Wang, Jie;Zhou, Yan;Cao, Dingguo;Li, Huimin;Li, Fuwei;Liu, Wei;Liu, Jie;Li, Dapeng;Han, Haixia;Sun, Yan;Lei, Qiuxia;Wang, Jie;Zhou, Yan;Cao, Dingguo;Li, Huimin;Li, Fuwei;Yan, Huaxiang
关键词：Lactobacillus salivarius; egg quality; intestinal morphology; cecal microbial community; laying hen
-
Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility
作者：Yang, Chunhong;Xiao, Yao;Wang, Xiuge;Wei, Xiaochao;Wang, Jinpeng;Gao, Yaping;Jiang, Qiang;Ju, Zhihua;Zhang, Yaran;Liu, Wenhao;Huang, Ning;Li, Yanqin;Gao, Yundong;Wang, Lingling;Huang, Jinming;Yang, Chunhong;Xiao, Yao;Wang, Xiuge;Wei, Xiaochao;Wang, Jinpeng;Gao, Yaping;Jiang, Qiang;Ju, Zhihua;Zhang, Yaran;Liu, Wenhao;Huang, Ning;Li, Yanqin;Gao, Yundong;Wang, Lingling;Huang, Jinming;Ju, Zhihua;Huang, Jinming;Huang, Jinming
关键词：Alternative splicing; dairy cattle; epigenetic regulation; PBRM1; spermatogenesis
-
Signatures of selection in indigenous Chinese cattle genomes reveal adaptive genes and genetic variations to cold climate
作者：Huang, Ning;Wang, Jinpeng;Jiang, Qiang;Ju, Zhihua;Huang, Jinming;Huang, Ning;Wang, Jinpeng;Ju, Zhihua;Wang, Xiuge;Zhang, Yaran;Xiao, Yao;Liu, Wenhao;Huang, Jinming;Huang, Ning;Lu, Shaoxiong;Zhao, Lihong
关键词：adaptive allele; cattle; cold adaptation; selective sweep; thermogenesis
