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Natural Horizontal Gene Transfer of Antimicrobial Resistance Genes in Campylobacter spp. From Turkeys and Swine

文献类型： 外文期刊

作者： Guernier-Cambert, Vanina 1 ; Trachsel, Julian 1 ; Maki, Joel 1 ; Qi, Jing 4 ; Sylte, Matthew J. 1 ; Hanafy, Zahra 5 ;

作者机构： 1.ARS, Food Safety & Enter Pathogens Res Unit, USDA, Natl Anim Dis Ctr, Ames, IA 50011 USA

2.ARS, Res Participat Program, Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA

3.Iowa State Univ, Interdept Microbiol Grad Program, Ames, IA USA

4.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan, Peoples R China

5.North Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC USA

关键词： antimicrobial resistance; Campylobacter; transformation; turkey; swine; animal experiment; co-culture experiment; horizontal gene transfer

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN：

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Antibiotic-resistant Campylobacter constitutes a serious threat to public health. The clonal expansion of resistant strains and/or the horizontal spread of resistance genes to other strains and species can hinder the clinical effectiveness of antibiotics to treat severe campylobacteriosis. Still, gaps exist in our understanding of the risks of acquisition and spread of antibiotic resistance in Campylobacter. While the in vitro transfer of antimicrobial resistance genes between Campylobacter species via natural transformation has been extensively demonstrated, experimental studies have favored the use of naked DNA to obtain transformants. In this study, we used experimental designs closer to real-world conditions to evaluate the possible transfer of antimicrobial resistance genes between Campylobacter strains of the same or different species (Campylobacter coli or Campylobacter jejuni) and originating from different animal hosts (swine or turkeys). This was evaluated in vitro through co-culture experiments and in vivo with dual-strain inoculation of turkeys, followed by whole genome sequencing of parental and newly emerged strains. In vitro, we observed four independent horizontal gene transfer events leading to the acquisition of resistance to beta-lactams (blaOXA), aminoglycosides [aph(2 '')-If and rpsL] and tetracycline [tet(O)]. Observed events involved the displacement of resistance-associated genes by a mutated version, or the acquisition of genomic islands harboring a resistance determinant by homologous recombination; we did not detect the transfer of resistance-carrying plasmids even though they were present in some strains. In vivo, we recovered a newly emerged strain with dual-resistance pattern and identified the replacement of an existing non-functional tet(O) by a functional tet(O) in the recipient strain. Whole genome comparisons allowed characterization of the events involved in the horizontal spread of resistance genes between Campylobacter following in vitro co-culture and in vivo dual inoculation. Our study also highlights the potential for antimicrobial resistance transfer across Campylobacter species originating from turkeys and swine, which may have implications for farms hosting both species in close proximity.

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