PRRSV nonstructural protein 11 degrades swine ISG15 by its endoribonuclease activity to antagonize antiviral immune response
文献类型： 外文期刊
作者： Jiang, Dandan 1 ; He, Maojuan 1 ; Sui, Chao 4 ; Wu, Xiangju 1 ; Hu, Yue 1 ; Cong, Xiaoyan 1 ; Li, Juntong 1 ; Du, Yijun 1 ; Qi, Jing 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Key Lab Livestock & Poultry Multi Omics MARA, Jinan, Peoples R China
2.Nanjing Agr Univ, Coll Vet Med, Key Lab Anim Dis Diagnost & Immunol,Minist Educ, MOE Int Joint Collaborat Res Lab Anim Hlth & Food, Nanjing, Peoples R China
3.Shandong Normal Univ, Coll Life Sci, Jinan, Peoples R China
4.Shandong Univ, Anim Lab, Qilu Hosp, Jinan, Peoples R China
关键词： PRRSV Nsp11; ISG15; Endoribonuclease; Autophagy; Antiviral response
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）
ISSN： 0378-1135
年卷期： 2023 年 280 卷
页码：
收录情况： SCI
摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped positive-stranded RNA virus which causes serious economic losses to pig industry worldwide. Type I IFN induces expression of interferon-stimulated genes 15 (ISG15) to inhibit virus replication. To survive in the host, PRRSV has evolved to antago-nize the antiviral response of ISGylation. Previous studies have reported that nonstructural protein 2 of PRRSV inhibits the ISGylation and antiviral function of ISG15 depending on its ovarian tumor (OTU) domain/papain-like protease domain (PLP2). However, whether there are other PRRSV proteins inhibiting ISGylation of cellular proteins is less well understood. In this study, we first found that PRRSV Nsp11 decreased ISGylation of cellular proteins. Meanwhile, the expression level of ISG15 was significantly inhibited by Nsp11. Further mechanistic studies demonstrated that the transcription of ISG15 was reduced by endoribonuclease activity of Nsp11. Finally, we found that the Nsp11-induced degradation of ISG15 was partially relied on autophagy-lysosome system. Taken together, PRRSV Nsp11 antagonizes the antiviral response of ISG15 by its endor-ibonuclease activity to promote PRRSV replication. Our results reveal a novel mechanism that PRRSV inhibits ISGylation of cellular proteins and impairs host innate immune response.
- 相关文献
作者其他论文 更多>>
-
GTPase activity of porcine Mx1 plays a dominant role in inhibiting the N-Nsp9 interaction and thus inhibiting PRRSV replication
作者：Hu, Yue;Wu, Xiangju;Tian, Yunfei;Jiang, Dandan;Ren, Jinrui;Li, Ziyong;Zhang, Quanfang;Cong, Xiaoyan;Li, Juntong;Du, Yijun;Qi, Jing;Ding, Xiuliang;Yoo, Dongwan;Miller, Laura C.;Lee, Changhee;Lee, Changhee
关键词：porcine reproductive and respiratory syndrome virus; interferons; antiviral agents; Mx1
-
The inhibitory effect of swine TAB1 on the replication of pseudorabies virus
作者：Xia, Xiujuan;Wu, Xiangju;Jiang, Dandan;Hu, Yue;Cong, Xiaoyan;Li, Juntong;Du, Yijun;Qi, Jing;Xia, Xiujuan;Dai, Meixue;Du, Yijun;Qi, Jing
关键词：STAB1; Pseudorabies virus; Inflammatory factors; Chemokines; IFN-beta
-
PRRSV NSP1α degrades TRIM25 through proteasome system to inhibit host antiviral immune response
作者：Zheng, Yuhang;Wang, Zhao;Du, Yijun;Qi, Jing;Huang, Juan;Lu, Yu;Lu, Yu;Jiang, Dandan;Wu, Xiangju;Hu, Yue;Cong, Xiaoyan;Li, Juntong;Du, Yijun;Qi, Jing;Sui, Chao;Wang, Zhao;Wang, Zhao;Lee, Changhee
关键词：PRRSV NSP1 alpha; TRIM25; Proteasome system; Antiviral response
-
Reprogramming viral immune evasion for a rational design of next-generation vaccines for RNA viruses
作者：Su, Chia-Ming;Rowland, Raymond R. R.;Yoo, Dongwan;Du, Yijun;Wang, Qiuhong;Wang, Qiuhong
关键词：type I interferons (IFNs); NF-kappa B (NF-kappa B); IFN antagonism; live-attenuated vaccine; veterinary vaccine; viral immune evasion; next-generation vaccines; veterinary virology
-
Sodium Butyrate Ameliorates Type 2 Diabetes-Related Sarcopenia Through IL-33-Independent ILC2s/IL-13/STAT3 Signaling Pathway
作者：Cao, Yuan;Li, Yulin;Han, Wenqiang;Jia, Xu;Zhu, Ping;Cao, Yuan;Li, Yulin;Han, Wenqiang;Jia, Xu;Zhu, Ping;Cao, Yuan;Li, Yulin;Han, Wenqiang;Jia, Xu;Zhu, Ping;Cao, Yuan;Li, Yulin;Han, Wenqiang;Jia, Xu;Zhu, Ping;Wei, Bin;Cong, Xiaoyan;Cong, Xiaoyan;Wang, Zhihao;Wang, Zhihao
关键词：diabetes mellitus; sarcopenia; ILC2s; sodium butyrate; IL-13
-
3C(pro) of FMDV inhibits type II interferon-stimulated JAK-STAT signaling pathway by blocking STAT1 nuclear translocation
作者：Wu, Xiangju;Sui, Chao;Hu, Yue;Jiang, Dandan;Li, Juntong;Cong, Xiaoyan;Hrabchenko, Nataliia;Du, Yijun;Qi, Jing;Chen, Lei;Du, Yijun;Qi, Jing;Yang, Fan;Miller, Laura C.;Lee, Changhee;Lee, Changhee
关键词：Foot-and-mouth disease virus (FMDV); 3C; IFN-gamma; JAK-STAT signaling pathway; STAT1; KPNA1
-
Swine NONO is an essential factor to inhibit pseudorabies virus infection
作者：Jiang, Dandan;Jiang, Chenlong;Jiang, Dandan;Sui, Chao;Wu, Xiangju;Hu, Yue;Cong, Xiaoyan;Li, Juntong;Du, Yijun;Qi, Jing;Lee, Changhee;Lee, Changhee
关键词：Pseudorabies virus; IFN-?; ISGs; SNONO
