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Selection Signature and CRISPR/Cas9-Mediated Gene Knockout Analyses Reveal ZC3H10 Involved in Cold Adaptation in Chinese Native Cattle

文献类型： 外文期刊

作者： Wang, Luyu 1 ; Gao, Yaping 1 ; Wang, Jinpeng 1 ; Huang, Ning 1 ; Jiang, Qiang 1 ; Ju, Zhihua 1 ; Yang, Chunhong 1 ; Wei, Xiaochao 1 ; Xiao, Yao 1 ; Zhang, Yaran 1 ; Yang, Ling 3 ; Huang, Jinming 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Key Lab Livestock & Poultry Multi MARA, Jinan 250100, Peoples R China

2.Shandong Key Lab Anim Dis Control Breeding & Repr, Jinan 250100, Peoples R China

3.Hebei Univ Engn, Sch Life Sci & Food Engn, Dept Anim Sci, Handan 056038, Peoples R China

关键词： cold adaptation; transcriptome; CRISPR; Cas9; cattle

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 10 期

页码：

收录情况： SCI

摘要： Cold stress is an important factor affecting cattle health, production performance, and reproductive efficiency. Understanding of the potential mechanism underlying genetic adaptation to local environments, particularly extreme cold environment, is limited. Here, by using FLK and hapFLK methods, we found that the Zinc finger CCCH-type containing 10 (ZC3H10) gene underwent positive selection in the Menggu, Fuzhou, Anxi, and Shigatse humped cattle breeds that are distributed in the cold areas of China. Furthermore, ZC3H10 expression significantly increased in bovine fetal fibroblast (BFF) cells at 28 degrees C for 4 h. ZC3H10 knockout BFFs were generated using CRISPR/Cas9. Wild and ZC3H10-deleted BFFs were treated at two temperatures and were divided into four groups (WT, wild and cultured at 38 degrees C; KO, ZC3H10(-/-) and 38 degrees C; WT_LT, wild, and 28 degrees C for 4 h; and KO_LT, ZC3H10(-/-) and 28 degrees C for 4 h. A total of 466, 598, 519, and 650 differently expressed genes (two-fold or more than two-fold changes) were identified by determining transcriptomic difference (KO_LT vs. KO, WT_LT vs. WT, KO vs. WT, and KO_LT vs. WT_LT, respectively). Loss of ZC3H10 dysregulated pathways involved in thermogenesis and immunity, and ZC3H10 participated in immunity-related pathways induced by cold stress and regulated genes involved in glucose and lipid metabolism and lipid transport (PLTP and APOA1), thereby facilitating adaptability to cold stress. Our findings provide a foundation for further studies on the function of ZC3H10 in cold stress and development of bovine breeding strategies for combatting the influences of cold climate.

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