Increases of ferrous iron oxidation activity and arsenic stressed cell growth by overexpression of Cyc2 in Acidithiobacillus ferrooxidans ATCC19859
文献类型： 外文期刊
作者： Liu, Wei 1 ; Lin, Jianqun 1 ; Pang, Xin 1 ; Mi, Shuang 1 ; Cui, Shuang 1 ; Lin, Jianqiang 1 ;
作者机构： 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China
2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Jinan, Peoples R China
3.Neusoft Co, Shenyang, Peoples R China
4.Shandong Univ, Sch Med, Jinan 250100, Peoples R China
关键词： Acidithiobacillus ferrooxidans; cyc2; conjugation; ferrous iron oxidation; bioleaching
期刊名称：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY （ 影响因子：2.431； 五年影响因子：2.124 ）
ISSN： 0885-4513
年卷期： 2013 年 60 卷 6 期
页码：
收录情况： SCI
摘要： Acidithiobacillus ferrooxidans plays an important role in bioleaching in reproducing the mineral oxidant of ferric iron (Fe3+) by oxidization of ferrous iron (Fe2+). The high-molecular-weight c-type cytochrome Cyc2 that is located in the external membrane is postulated as the first electron carrier in the Fe2+ oxidation respiratory pathway of A. ferrooxidans. To increase ferrous iron oxidation activity, a recombinant plasmid pTCYC2 containing cyc2 gene under the control of Ptac promoter was constructed and transferred into A. ferrooxidansATCC19859. The transcriptional level of cyc2 gene was increased by 2.63-fold and Cyc2 protein expression was observed in the recombinant strain compared with the control. The ferrous iron oxidation activity and the arsenic stressed cell growth of the recombinant strain were also elevated. (C) 2013 International Union of Biochemistry and Molecular Biology, Inc. Volume 60, Number 6, Pages 623-628, 2013
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