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Characterization of miRNA profiles in the mammary tissue of dairy cattle in response to heat stress

文献类型： 外文期刊

作者： Li, Qiuling 1 ; Yang, Chunhong 2 ; Du, Juan 1 ; Zhang, Baogui 3 ; He, Ying 4 ; Hu, Qimeng 1 ; Li, Meiru 1 ; Zhang, Yiming 1 ; Wang, Changfa 2 ; Zhong, Jifeng 2 ;

作者机构： 1.Langfang Normal Univ, Coll Life Sci, Edible & Med Fungi Res & Dev Ctr, Langfang 065000, Peoples R China

2.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan 250100, Shandong, Peoples R China

3.Yongqing Anim Husb & Vet Bur, Yongqing 065600, Peoples R China

4.Dachang Cty Anim Hlth Supervis Inst, Dachang 065300, Peoples R China

关键词： Dairy cattle; Heat stress; Mammary gland; MicroRNA

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2018 年 19 卷

页码：

收录情况： SCI

摘要： BackgroundMicroRNAs (miRNAs) are a class of small noncoding RNAs that play important roles in the regulation of gene expression. However, the role of miRNAs in bovine mammary gland responses to heat stress is not well understood.ResultsIn the present study, we performed a deep RNA sequencing analysis to identify miRNAs associated with the heat stress potential of the bovine mammary gland. We identified 27 miRNAs that were differentially expressed significantly between the mammary tissue of Holstein cattle heat stress and normal conditions. Twenty miRNAs had higher expression in the mammary tissue of heat-stressed Holstein cattle. The seven highest differentially expressed candidate miRNAs (bta-miR-21-5p, bta-miR-99a-5p, bta-miR-146b, bta-miR-145, bta-miR-2285t, bta-miR-133a, and bta-miR-29c) identified by deep RNA sequencing were additionally evaluated by stem-loop qPCR. Enrichment analyses for targeted genes revealed that the major differences between miRNAs expression in the mammary gland of heat-stressed versus control were associated with the regulation of Wnt, TGF-, MAPK, Notch, and JAK-STAT.ConclusionsThese data indicated that the differentially expressed miRNAs identified in this study may act as dominant regulators during heat stress. We might reduce heat stress damage of Holstein cows by up-regulating or down-regulating these differentially expressed miRNAs.

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[1]Characterization of miRNA profiles in the mammary tissue of dairy cattle in response to heat stress. Li, Qiuling,Du, Juan,Hu, Qimeng,Li, Meiru,Zhang, Yiming,Li, Qiuling,Yang, Chunhong,Wang, Changfa,Zhong, Jifeng,Zhang, Baogui,He, Ying. 2018

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