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The Plastidial DIG5 Protein Affects Lateral Root Development by Regulating Flavonoid Biosynthesis and Auxin Transport in Arabidopsis

文献类型： 外文期刊

作者： Liu, Wei 1 ; Chen, Tao 3 ; Liu, Yajie 4 ; Quang Tri Le 5 ; Wang, Ruigang 1 ; Lee, Hojoung 5 ; Xiong, Liming 1 ;

作者机构： 1.Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA

2.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan 250100, Peoples R China

3.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 311121, Peoples R China

4.Hong Kong Baptist Univ, Dept Biol, Kowloon Tang, Hong Kong, Peoples R China

5.Korea Univ, Coll Life Sci & Biotechnol, Dept Plant Biotechnol, Seoul 136713, South Korea

6.Inner Mongolia Agr Univ, Inner Mongolia Key Lab Plant Stress Physiol & Mol, Hohhot 010010, Peoples R China

7.Chinese Univ Hong Kong, State Key Lab Agribiotechnol, Hong Kong, Peoples R China

关键词： lateral roots; root system architecture; flavonoid; polar auxin transport; chloroplast; retrograde signaling; tRNA adenosine deaminase arginine

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 18 期

页码：

收录情况： SCI

摘要： To reveal the mechanisms underlying root adaptation to drought stress, we isolated and characterized an Arabidopsis mutant, dig5 (drought inhibition of lateral root growth 5), which exhibited increased sensitivity to the phytohormone abscisic acid (ABA) for the inhibition of lateral root growth. The dig5 mutant also had fewer lateral roots under normal conditions and the aerial parts were yellowish with a lower level of chlorophylls. The mutant seedlings also displayed phenotypes indicative of impaired auxin transport, such as abnormal root curling, leaf venation defects, absence of apical hook formation, and reduced hypocotyl elongation in darkness. Auxin transport assays with [H-3]-labeled indole acetic acid (IAA) confirmed that dig5 roots were impaired in polar auxin transport. Map-based cloning and complementation assays indicated that the DIG5 locus encodes a chloroplast-localized tRNA adenosine deaminase arginine (TADA) that is involved in chloroplast protein translation. The levels of flavonoids, which are naturally occurring auxin transport inhibitors in plants, were significantly higher in dig5 roots than in the wild type roots. Further investigation showed that flavonoid biosynthetic genes were upregulated in dig5. Introduction of the flavonoid biosynthetic mutation transparent testa 4 (tt4) into dig5 restored the lateral root growth of dig5. Our study uncovers an important role of DIG5/TADA in retrogradely controlling flavonoid biosynthesis and lateral root development. We suggest that the DIG5-related signaling pathways, triggered likely by drought-induced chlorophyll breakdown and leaf senescence, may potentially help the plants to adapt to drought stress through optimizing the root system architecture.

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[1]The Plastidial DIG5 Protein Affects Lateral Root Development by Regulating Flavonoid Biosynthesis and Auxin Transport in Arabidopsis. Liu, Wei,Wang, Ruigang,Xiong, Liming,Liu, Wei,Chen, Tao,Liu, Yajie,Xiong, Liming,Quang Tri Le,Lee, Hojoung,Wang, Ruigang,Xiong, Liming. 2022

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