文献类型： 外文期刊

作者： Agarwal, Gaurav ¹ ; Clevenger, Josh ⁴ ; Pandey, Manish K. ³ ; Wang, Hui ¹ ; Shasidhar, Yaduru ³ ; Chu, Ye ⁶ ; Fountain, Jake C. ¹ ; Choudhary, Divya ¹ ; Culbreath, Albert K. ² ; Liu, Xin ⁸ ; Huang, Guodong ⁸ ; Wang, Xingjun ⁹ ; Deshmukh, Rupesh ¹⁰ ; Holbrook, C. Corley ¹¹ ; Bertioli, David J. ⁵ ; Ozias-Akins, Peggy ⁶ ; Jackson, Scott A. ⁵ ; Varshney, Rajeev K. ³ ; Guo, Baozhu ¹ ;

作者机构： 1.USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA

2.Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA

3.Int Crops Res Inst Semi Arid Trop, Ctr Excellence Genom & Syst Biol, Hyderabad, Telangana, India

4.Mars Wrigley Confectionery, Ctr Appl Genet Technol, Athens, GA USA

5.Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA

6.Univ Georgia, Dept Hort, Tifton, GA USA

7.Univ Georgia, Inst Plant Breeding & Genom, Tifton, GA USA

8.BGI Shenzhen, Shenzhen, Peoples R China

9.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan, Shandong, Peoples R China

10.Univ Laval, Quebec City, PQ, Canada

11.USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA

关键词： whole-genome resequencing; high-density genetic map; quantitative trait loci; early leaf spot; late leaf spot; Tomato spotted wilt virus

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2018 年 16 卷 11 期

页码：

收录情况： SCI

摘要： Whole-genome resequencing (WGRS) of mapping populations has facilitated development of high-density genetic maps essential for fine mapping and candidate gene discovery for traits of interest in crop species. Leaf spots, including early leaf spot (ELS) and late leaf spot (LLS), and Tomato spotted wilt virus (TSWV) are devastating diseases in peanut causing significant yield loss. We generated WGRS data on a recombinant inbred line population, developed a SNP-based high-density genetic map, and conducted fine mapping, candidate gene discovery and marker validation for ELS, LLS and TSWV. The first sequence-based high-density map was constructed with 8869 SNPs assigned to 20 linkage groups, representing 20 chromosomes, for the 'T' population (Tifrunner x GT-C20) with a map length of 3120 cM and an average distance of 1.45 cM. The quantitative trait locus (QTL) analysis using high-density genetic map and multiple season phenotyping data identified 35 main-effect QTLs with phenotypic variation explained (PVE) from 6.32% to 47.63%. Among major-effect QTLs mapped, there were two QTLs for ELS on B05 with 47.42% PVE and B03 with 47.38% PVE, two QTLs for LLS on A05 with 47.63% and B03 with 34.03% PVE and one QTL for TSWV on B09 with 40.71% PVE. The epistasis and environment interaction analyses identified significant environmental effects on these traits. The identified QTL regions had disease resistance genes including R-genes and transcription factors. KASP markers were developed for major QTLs and validated in the population and are ready for further deployment in genomics-assisted breeding in peanut.