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The N-mannosyltransferase gene BbAlg9 contributes to cell wall integrity, fungal development and the pathogenicity of Beauveria bassiana

文献类型： 外文期刊

作者： Song, Ji-Zheng 1 ; Yin, Ya-Ping 1 ; Cheng, Wen 3 ; Liu, Jia-Hua 2 ; Hu, Shun-Juan 2 ; Qiu, Lei 2 ; Wang, Juan-Juan 1 ;

作者机构： 1.Univ Jinan, Sch Biol Sci & Technol, Jinan 250022, Shandong, Peoples R China

2.Qilu Univ Technol, Shandong Acad Sci, State Key Lab Biobased Mat & Green Papermaking, Jinan, Peoples R China

3.Shandong Acad Agr Sci, Maize Res Inst, Jinan, Peoples R China

关键词： Entomopathogenic fungi; N-mannosyltransferase; Alg9; Virulence

期刊名称：FUNGAL BIOLOGY （ 影响因子：3.099； 五年影响因子：3.364 ）

ISSN： 1878-6146

年卷期： 2021 年 125 卷 10 期

页码：

收录情况： SCI

摘要： The mannosyltransferase Alg9 plays a vital role in N-linked protein glycosylation in Saccharomyces cerevisiae, but its function in most filamentous fungi is not clear. The present study characterized BbAlg9 (an ortholog of S. cerevisiae Alg9) in Beauveria bassiana to determine the roles of N-mannosyltransferase in biological control potential of the filamentous entomopathogenic fungus. The disruption of BbAlg9 led to slower fungal growth in media with various nutrition compositions. The conidiation of DBbAlg9 was less than that of the wild type from the third to the fifth day but showed no significant difference on the sixth day, suggesting that BbAlg9 affects the development of conidia rather than conidial yield of late stage. DBbAlg9 showed defects in conidial germination, multiple stress tolerances and the yield of blastospores, with altered size and density, and virulence in hosts infected via the immersion and injection methods. The deletion of BbAlg9 resulted in defects in cell wall integrity, including increased mannoprotein and glucan content and decreased chitin content, which were accompanied by transcriptional activation or suppression of genes related to cell wall component biosynthesis. Notably, deletion of the N-mannosyltransferase BbAlg9 altered the transcription levels of O-mannosyltransferase genes (Pmt and Ktr family). These data show that BbAlg9 is involved in the fungal development, conidial stress tolerance, cell wall integrity and virulence of B. bassiana. (c) 2021 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

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