Isolation and characterization of a 295-bp strong promoter of maize high-affinity phosphate transporter gene ZmPht1; 5 in transgenic Nicotiana benthamiana and Zea mays
文献类型： 外文期刊
作者： Li, Wendi 1 ; Liu, Baiyu 1 ; Zhao, Mengsha 1 ; Zhang, Ke 1 ; He, Qiuxia 2 ; Zhao, Xiangyu 3 ; Cheng, Wen 4 ; Ding, Zhaohu 1 ;
作者机构： 1.Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Peoples R China
2.Qilu Univ Technol, Biol Inst, Shandong Acad Sci, Jinan, Shandong, Peoples R China
3.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
4.Shandong Acad Agr Sci, Maize Inst, Jinan, Shandong, Peoples R China
关键词： Crop improvement; Genetic transformation; GUS analysis; Pi-starvation-inducible promoter; Transgenic breeding
期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）
ISSN： 0032-0935
年卷期： 2020 年 251 卷 6 期
页码：
收录情况： SCI
摘要： Main conclusion The small 295-bp ZmPht1; 5 promoter is sufficient to drive high-intensity expression of target genes, especially under phosphate deprivation conditions, and is therefore useful for crop improvement via transgenic techniques. Phosphate (Pi) deficiency has become a major challenge and limiting factor in world agricultural production. Manipulating the gene expression using appropriate promoters to improve the Pi absorption and utilization efficiency of crops could reduce the requirement for Pi fertilizers. In the study, a 295-bp strong promoter (M2P-7) of maize high-affinity phosphate transporter ZmPht1; 5 was isolated and functionally validated in transgenic Nicotiana benthamiana and maize by analyzing the ZmPht1; 5 promoter (M2P-1) and its 5 ' truncated variants (M2P-2 similar to M2P-8) in different sizes under normal and Pi-deprivation conditions. The M2P-7 displayed the highest promoter activities among 5 ' truncated fragments in all tested tissues of transgenic Nicotiana benthamiana at different development stages, which was 1.5 and 3 times higher than the well-used CaMV35S promoter under normal and Pi-deprivation conditions, respectively. In maize, the M2P-7 promoter activity was comparable to the maize ubiquitin1 promoter widely used in monocots under normal condition, which was about 1.3 times that of the ubiquitin1 promoter under Pi-deprivation environments. Moreover, the M2P-7 fragment is only 295 bp in length, thus reducing the construct size, and is therefore beneficial for genetic transformation. Thus, the small promoter M2P-7 of plant origin could be of great use for monocotyledonous and dicotyledonous crop improvement via transgenic techniques based on its promoter activities, expression patterns and small size.
- 相关文献
作者其他论文 更多>>
-
Membralin is required for maize development and defines a branch of the endoplasmic reticulum-associated degradation pathway in plants
作者：Liu, Baiyu;Zhang, Ke;Qi, Shoumei;Jin, Zhe;Chen, Donghua;Zhang, Wei;Zhang, Kewei;Li, Kunpeng;Xu, Changzheng;He, Qiuxia;Cheng, Wen;Ding, Zhaohua;Zhao, Xiangyu
关键词：endoplasmic reticulum-associated degradation; maize; membralin; plant development; unfold protein response
-
Optimizing root system architecture to improve cotton drought tolerance and minimize yield loss during mild drought stress
作者：Guo, Congcong;Bao, Xiaoyuan;Sun, Hongchun;Zhu, Lingxiao;Zhang, Yongjiang;Zhang, Ke;Bai, Zhiying;Liu, Xiaoqing;Li, Anchang;Dong, Hezhong;Liu, Liantao;Li, Cundong;Dong, Hezhong;Zhan, Lijie;Zhu, Jijie
关键词：Root system architecture; Drought stress; Cotton; Drought tolerance; Yield
-
Research Progress on the Mechanism of Salt Tolerance in Maize: A Classic Field That Needs New Efforts
作者：Li, Jiawei;Zhu, Qinglin;Jiao, Fuchao;Zhang, Haiyan;Zhang, Yumei;Chen, Jingtang;Wang, Ming;Jiao, Fuchao;Zhang, Haiyan;Zhang, Yumei;Chen, Jingtang;Wang, Ming;Yan, Zhenwei;Ding, Zhaohua;Mu, Chunhua;Liu, Xia;Li, Yan
关键词：maize; salt stress; osmolyte; antioxidant enzymes; reactive oxygen species
-
Effects of Mineral-Based Potassium Humate on Cadmium Accumulation in Rice (Oryza sativa L.) under Three Levels of Cadmium-Contaminated Alkaline Soils
作者：Li, Shuangshuang;Zhang, Ke;Bai, Liyong;Dai, Jiulan;Huang, Xianmin;Li, Guangxian;He, Huan;Chen, Shibao
关键词：mineral-based potassium humate; cadmium; rice; antioxidant enzymes; bioavailability; photosynthetic characteristics
-
A large deletion conferring pale green leaves of maize
作者：Yao, Guoqi;Zhang, Hua;Leng, Bingying;Cao, Bing;Shan, Juan;Yan, Zhenwei;Guan, Haiying;Cheng, Wen;Liu, Xia;Mu, Chunhua;Yao, Guoqi;Zhang, Hua;Leng, Bingying;Cao, Bing;Shan, Juan;Yan, Zhenwei;Guan, Haiying;Cheng, Wen;Liu, Xia;Mu, Chunhua;Yao, Guoqi;Zhang, Hua;Leng, Bingying;Cao, Bing;Shan, Juan;Yan, Zhenwei;Guan, Haiying;Cheng, Wen;Liu, Xia;Mu, Chunhua;Yao, Guoqi;Zhang, Hua;Leng, Bingying;Cao, Bing;Shan, Juan;Yan, Zhenwei;Guan, Haiying;Cheng, Wen;Liu, Xia;Mu, Chunhua;Liu, Xia
关键词：Maize; Pgl; Map based cloning; Large deletion
-
The effect of exogenous melatonin on root growth and lifespan and seed cotton yield under drought stress
作者：Zhu, Lingxiao;Li, Anchang;Sun, Hongchun;Liu, Xiaoqing;Guo, Congcong;Zhang, Yongjiang;Zhang, Ke;Bai, Zhiying;Dong, Hezhong;Liu, Liantao;Li, Cundong;Li, Ping;Dong, Hezhong
关键词：Cotton; Drought; Melatonin; Root growth; Root lifespan; Yield
-
Cultivar-specific response of rhizosphere bacterial community to uptake of cadmium and mineral elements in rice (Oryza sativa L.)
作者：Li, Shuangshuang;Chen, Yihui;Lv, Cheng;Bai, Liyong;Zhang, Ke;He, Huan;Dai, Jiulan;Li, Guangxian;Huang, Xianmin;Dai, Jiulan
关键词：Cadmium; Japonica rice cultivar; Mineral element; Rhizosphere soil; Microbial community
