Fucoxanthin's Optimization from Undaria pinnatifida Using Conventional Heat Extraction, Bioactivity Assays and In Silico Studies
文献类型： 外文期刊
作者： Lourenco-Lopes, Catarina 1 ; Fraga-Corral, Maria 1 ; Soria-Lopez, Anton 1 ; Nunes-Estevez, Bernabe 1 ; Barral-Martinez, Marta 1 ; Silva, Aurora 1 ; Li, Ningyang 4 ; Liu, Chao 5 ; Simal-Gandara, Jesus 1 ; Prieto, Miguel A. 1 ;
作者机构： 1.Univ Vigo, Fac Food Sci & Technol, Analyt & Food Chem Dept, Nutr & Bromatol Grp, Ourense Campus, E-32004 Orense, Spain
2.Inst Politecn Braganca, Ctr Invest Montanha CIMO, Campus Santa Apolonia, P-5300253 Braganca, Portugal
3.Inst Politecn Porto, Inst Super Engn Porto, REQUIMTE LAQV, Rua Dr Antonio Bernardino de Almeida 431, P-4200072 Porto, Portugal
4.Shandong Agr Univ, Coll Food Sci & Engn, Key Lab Food Proc Technol & Qual Control Shandong, Tai An 271002, Shandong, Peoples R China
5.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Key Lab Novel Food Resources Proc, Minist Agr & Rural Affairs, Jinan 250000, Peoples R China
6.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Key Lab Agroprod Proc, Technol Shandong Prov, Jinan 250000, Peoples R China
关键词： fucoxanthin; conventional heat extraction; Undaria pinnatifida; kinetics; antioxidant activity; neuroprotective activity; antimicrobial activity; in silico studies; docking
期刊名称：ANTIOXIDANTS （ 影响因子：7.675； 五年影响因子：7.886 ）
ISSN：
年卷期： 2022 年 11 卷 7 期
页码：
收录情况： SCI
摘要： Brown macroalgae are a potential source of natural pigments. Among them, Undaria pinnatifida is recognized for its high concentration of fucoxanthin (Fx), which is a pigment with a wide range of bioactivities. In this study, three independent parameters were optimized for conventional heat extraction (CHE) to maximize the recovery of Fx from Undaria pinnatifida. Optimal conditions (temperature = 45 degrees C, solvent = 70%, and time = 61 min) extracted 5.1 mg Fx/g dw. Later, the bioactivities of the Fx-rich extracts (antioxidant, antimicrobial, and neuroprotective) were assessed using in vitro and in silico approaches. In vitro assays indicated that Fx has a strong antioxidant capacity and even stronger antimicrobial activity against gram-positive bacteria. This data was supported in silico where Fx established a high binding affinity to DR, a Staphylococcus aureus protein, through aa ALA-8, LEU-21, and other alkane interactions. Finally, the in vitro enzymatic inhibition of AChE using Fx, was further supported using docking models that displayed Fx as having a high affinity for aa TYR72 and THR 75; therefore, the Fx extraction behavior explored in this work may reduce the costs associated with energy and solvent consumption. Moreover, this paper demonstrates the efficiency of CHE when recovering high amounts of Fx from Undaria pinnatifida. Furthermore, these findings can be applied in different industries.
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