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A novel nicotinamide adenine dinucleotide control strategy for increasing the cell density of Haemophilus parasuis

文献类型： 外文期刊

作者： Cheng, Likun 1 ; Li, Feng 1 ; Li, Shuguang 1 ; Lin, Chuwen 1 ; Fu, Qiang 1 ; Yin, Huanhuan 1 ; Tian, Fengrong 1 ; Qu, Gua 1 ;

作者机构： 1.Shandong Binzhou Anim Sci & Vet Med Acad, Postdoctoral Sci Res Workstn, Binzhou, Peoples R China

2.Shandong Lvdu Biosci & Technol Co Ltd, Key Lab Binzhou High Cell Dens Fermentat, Binzhou, Peoples R China

3.Shandong Acad Agr Sci, Inst Poultry, Jinan, Shandong, Peoples R China

关键词： control strategy; Haemophilus parasuis; linear relationship; nicotinamide adenine dinucleotide; real-time monitoring

期刊名称：BIOTECHNOLOGY PROGRESS （ 影响因子：2.681； 五年影响因子：2.691 ）

ISSN： 8756-7938

年卷期： 2019 年 35 卷 3 期

页码：

收录情况： SCI

摘要： Haemophilus parasuis is the causative agent of Glasser's disease and is a major source of economic losses in the swine industry each year. To enhance the production of an inactivated vaccine against H. parasuis, the availability of nicotinamide adenine dinucleotide (NAD) must be carefully controlled to ensure a sufficiently high cell density of H. parasuis. In the present study, the real-time viable cell density of H. parasuis was calculated based on the capacitance of the culture. By assessing the relationship between capacitance and viable cell density/NAD concentration, the NAD supply rate could be adjusted in real time to maintain the NAD concentration at a set value based on the linear relationship between capacitance and NAD consumption. The linear relationship between cell density and addition of NAD indicated that 7.138 x 10(9) NAD molecules were required to satisfy per cell growth. Five types of NAD supply strategy were used to maintain different NAD concentration for H. parasuis cultivation, and the results revealed that the highest viable cell density (8.57, OD600) and cell count (1.57 x 10(10) CFU/mL) were obtained with strategy III (NAD concentration maintained at 30 mg/L), which were 1.46- and 1.45- times more, respectively, than cultures with using NAD supply strategy I (NAD concentration maintained at 10 mg/L). An extremely high cell density of H. parasuis was achieved using this NAD supply strategy, and the results demonstrated a convenient and reliable method for determining the real-time viable cell density relative to NAD concentration. Moreover, this method provides a theoretical foundation and an efficient approach for high cell density cultivation of other auxotroph bacteria.

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