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Study on host-seeking behavior and chemotaxis of entomopathogenic nematodes using Pluronic F-127 gel

文献类型： 外文期刊

作者： Li, Chunjie 1 ; Zhou, Xianhong 2 ; Lewis, Edwin E. 3 ; Yu, Yi 2 ; Wang, Congli 1 ;

作者机构： 1.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Harbin 150081, Heilongjiang, Peoples R China

2.Shandong Acad Agr Sci, Inst Plant Protect, Shandong Key Lab Plant Virol, Jinan 250100, Shandong, Peoples R China

3.Univ Idaho, Dept Entomol Plant Pathol & Hematol, Moscow, ID 83844 USA

关键词： Entomopathogenic nematodes; Tritrophic interactions; Host-seeking behavior; Pluronic gel; Acetic acid attraction

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN： 0022-2011

年卷期： 2019 年 161 卷

页码：

收录情况： SCI

摘要： Pluronic F-127 gel (PF127) has proven to be a powerful medium in which to study host-finding behavior and chemotaxis for plant-parasitic nematodes. Pluronic gel can also be used to study host-habitat seeking behavior of entomopathogenic nematodes (EPN), which are natural enemies of root-feeding insect pests. In this study, PF127 was used to study tritrophic interactions among EPNs, host-habitat roots and insects. We also tested whether EPN aggregated to acetic acid (pH gradient) which mimicked the conditions near the roots. The chive root gnat Bradysia odoriphaga alone significantly attracted more nematodes than chive roots alone or the combination of roots plus insects. The attractiveness of B. odoriphaga differed (3.7-15.4%) among all tested species/strains of EPNs. In addition, we found that Heterorhabditis spp. and Steinernema spp. infective juveniles responded to pH gradients formed by acetic acid in Pluronic gel. The preferred pH ranges for strains of H. bacteriophora and H. megidis were from 4.32-5.04, and from 5.37-6.92 for Steinernema species, indicating that Heterorhabditis spp. prefer low pH conditions than Steinemema species. A narrow pH gradient between 6.84 and 7.05 was detected around chive root tips in which EPN was attracted. These results suggest that Pluronic gel can be broadly used for the study of host or host-habitat seeking behaviors and chemotaxis of nematodes.

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