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Identification of miRNAs and their targets in regulating tuberous root development in radish using small RNA and degradome analyses

文献类型： 外文期刊

作者： Liu, Chen 1 ; Liu, Xianxian 1 ; Xu, Wenling 1 ; Fu, Weimin 1 ; Wang, Fengde 1 ; Gao, Jianwei 2 ; Li, Qiaoyun; Zhang, Z 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Vegetables & Flowers, Natl Vegetable Improvement Ctr, Shandong Branch,Shandong Key Lab Greenhouse Veget, Jinan 250100, Shandong, Peoples R China

2.Shandong Acad Agr Sci, Inst Vegetables & Flowers, Natl Vegetable Improvement Ctr, Shandong Branch,Shandong Key Lab Greenhouse Veget, Jinan 250100, Shandon

关键词： Raphanus sativus; Tuberous root; Cortex splitting; microRNA; Degradome

期刊名称：3 BIOTECH （ 影响因子：2.406； 五年影响因子：3.203 ）

ISSN： 2190-572X

年卷期： 2018 年 8 卷 7 期

页码：

收录情况： SCI

摘要： High-throughput small RNA sequencing and degradome analysis were used in this study to thoroughly investigate the role of miRNA-mediated regulatory network in tuberous root development of radish. Samples from the early seedling stage (RE) and the cortex splitting stage (RL) were used for the construction of six small RNA libraries and one degradome library. A total of 518 known and 976 novel miRNAs were identified, of which, 338 known and 18 novel miRNAs were expressed in all six libraries, respectively. A total of 52 known and 57 novel miRNAs were identified to be significantly differentially expressed between RE and RL, and 195 mRNAs were verified to be the targets of 194 miRNAs by degradome sequencing. According to the degradome analysis, 11 differentially expressed miRNAs had miRNA-mRNA targets, and 13 targets were identified for these 11 miRNAs. Of the 13 miRNA-mRNA targets, 4 genes (RSG11079.t1, RSG11844.t1, RSG16775.t1, and RSG42419.t1) were involved in hormone-mediated signaling pathway, 2 gens (RSG11079.t1 and RSG16775.t1) were related to postembryonic root development, and 1 gene (RSG23799.t1) was involved in anatomical structure morphogenesis, according to the GO function analysis for biological process. Target Genes participated in these processes are important candidates for further studies. This study provides valuable information for a better understanding of the molecular mechanisms involved in radish tuberous root formation and development.

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[1]Identification of miRNAs and their targets in regulating tuberous root development in radish using small RNA and degradome analyses. Liu, Chen,Liu, Xianxian,Xu, Wenling,Fu, Weimin,Wang, Fengde,Gao, Jianwei,Li, Qiaoyun,Zhang, Zhigang,Li, Jingjuan,Wang, Shufen. 2018

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